公开(公告)号：US20230333105A1

公开(公告)日：2023-10-19

申请号：US18133908

申请日：2023-04-12

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Adam Thomas Wright ,  Ping He

IPC: G01N33/569 ,  G01N15/14 ,  G01N33/533

CPC classification number: G01N33/56966 ,  G01N15/1434 ,  G01N33/533 ,  G01N2015/1006

Abstract: Methods of producing a plurality of distinguishably fluorescently barcoded particle, e.g., cellular, bead, etc., samples, e.g., for use in the multiplex flow cytometric workflows, are provided. Aspects of the methods include: providing a plurality of particle, e.g., cellular, bead, etc., samples; and labeling different particle, e.g., cellular, bead, etc., samples of the plurality with unique fluorescent barcodes, wherein a given fluorescent barcode comprises one or more fluorescently labeled specific binding members that specifically bind to a particle marker. Also provided are compositions for practicing methods of the invention.

公开(公告)号：US20250066843A1

公开(公告)日：2025-02-27

申请号：US18728980

申请日：2023-02-06

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Katherine Lazaruk ,  Tracy Campbell ,  Dennis Prosen ,  Adam Thomas Wright ,  Hye-Won Song

IPC: C12Q1/6851 ,  C12Q1/6804 ,  C12Q1/6834

Abstract: Disclosed herein include systems, methods, compositions, and kits for reducing non-specific noise-causing oligonucleotides in library preparations. Capture particles present in PCR reactions that are derived from partitions that did not contain a cell (e.g., noise capture particles) provide a significant source of non-specific nucleic acid and noise in single cell multiomics library preparations. In some embodiments of the methods provided herein, said noise capture particles are removed by a variety of approaches.

公开(公告)号：US20240369545A1

公开(公告)日：2024-11-07

申请号：US18686824

申请日：2022-08-30

Applicant: Becton, Dickinson and Company

Inventor： Adam Thomas Wright ,  Jody Martin ,  Hye-Won Song ,  Samatha Vadrevu

IPC: G01N33/53 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68

Abstract: Antibodies attached to oligonucleotides can bind to proteins of cells. These oligonucleotides can be released and captured by solid support oligonucleotides attached to solid supports. A detection oligonucleotide can hybridize to the captured oligonucleotides forming a sandwich of solid support oligonucleotide-captured oligonucleotide-detection oligonucleotide. The detection oligonucleotide and the captured oligonucleotides it binds to can be quantified by, for example, the fluorescent intensity of second detectable moieties (e.g., dye(s)) on the detection oligonucleotide. The identity of the protein quantified can be determined by, for example, the fluorescent intensity of first detectable moieties (e.g., dye(s)) on the solid support with the sandwich of solid support oligonucleotide-captured oligonucleotide-detection oligonucleotide.