公开(公告)号：US20190234863A1

公开(公告)日：2019-08-01

申请号：US16382056

申请日：2019-04-11

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Eric Diebold ,  Keegan Owsley ,  Jonathan Lin

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1429 ,  G01N15/147 ,  G01N21/6402 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1447 ,  G01N2015/149 ,  G01N2021/6439

Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

公开(公告)号：US20250093671A1

公开(公告)日：2025-03-20

申请号：US18954285

申请日：2024-11-20

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Eric Diebold ,  Jorge Manzarraga ,  Jizuo Zou

IPC: G02B27/09 ,  G01N15/1434 ,  G02B3/00 ,  G02B19/00

Abstract: Aspects of the present disclosure include systems with multiple lasers having modified beam profiles. Systems according to certain embodiments include a first laser that produces a first beam of light, a second laser that produces a second beam of light and a beam shaping component that receives the first beam of light and the second beam of light at substantially the same position from different angles of incidence and is configured to generate from the first beam of light and the second beam of light an output beam of light having a predetermined intensity profile along a horizontal axis. Methods for irradiating a sample in a flow stream with the output beam of light are also described. Kits having one or more lasers and a beam shaping component configured to generate from a first beam of light and a second beam of light an output beam of light having a predetermined intensity profile along a horizontal axis are also provided.

公开(公告)号：US20240377311A1

公开(公告)日：2024-11-14

申请号：US18657633

申请日：2024-05-07

Applicant: Becton, Dickinson and Company

Inventor： Aaron Jacob Tyznik ,  Aaron Jacob Middlebrook ,  Eric Diebold ,  Keegan Owsley ,  Shirley Shi ,  Woodrow E. Lomas

IPC: G01N15/1434

Abstract: Aspects of the present disclosure include methods for calculating one or more cell parameters of cells in a sample. Methods according to certain embodiments include irradiating a sample comprising cells in a flow stream with frequency-modulated beams of light, measuring light from the cells in the sample, generating image data of the cells from the measured light and calculating one or more cell parameters based on the generated image data. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also described. Non-transitory computer readable storage media are also provided.