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公开(公告)号：US20220089877A1
公开(公告)日：2022-03-24
申请号：US17531067
申请日：2021-11-19
发明人： Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
摘要： Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20200048469A1
公开(公告)日：2020-02-13
申请号：US16523871
申请日：2019-07-26
摘要： Polymeric tandem dyes are provided. The polymeric tandem dyes include a narrow emission fluorophore configured as a pendant acceptor in energy-receiving proximity to a donor water solvated light harvesting multichromophore. The narrow emission acceptor fluorophores of the present disclosure are capable of providing polymeric tandem dyes having a variety of narrow emission spectrums suitable for multiplexing. Also provided are compositions for multicolor fluorescent signal generation that include two or more polymeric tandem dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20230392071A1
公开(公告)日：2023-12-07
申请号：US18201940
申请日：2023-05-25
发明人： Glenn Bartholomew , David Moureau
IPC分类号： C09K11/06 , C08G69/48 , C09B69/10 , G01N33/542 , G01N33/533
CPC分类号： C09K11/06 , C08G69/48 , C09B69/10 , C09K2211/1425 , G01N33/533 , C09K2211/1491 , C09K2211/1466 , G01N33/542
摘要： Green excitable tandem dyes are provided. Tandem dyes of embodiments of the invention include: a non-conjugated polymeric backbone; one or more green excitable donor fluorophores linked to the non-conjugated polymeric backbone; and one or more acceptor fluorophores linked to the non-conjugated polymeric backbone; where the donor and acceptor fluorophores are in energy transfer relationship. Also provided are methods of making and using the tandem dyes, as well as kits that include the dyes and find use in embodiments of the methods.
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公开(公告)号：US20220348770A1
公开(公告)日：2022-11-03
申请号：US17699790
申请日：2022-03-21
发明人： Glenn Bartholomew , Brian Wall , Jon Hollinger , Yongchao Liang
IPC分类号： C09B69/10
摘要： Water-soluble dipyrromethene-based dyes are provided herein. In certain cases, the water-soluble dye is a dipyrromethene-boron dye. Embodiments of the subject water-soluble dipyrromethene-based dyes have a narrow absorption maxima in the yellow green range close to or at the 561 nm laser line, and are suitable for use in tandem dye systems. Embodiments of the subject dyes efficiently transfer energy to an acceptor chromophore while a second dipyrromethene-based dye of the same or different chemical structure is within energy-receiving proximity therewith. Also provided herein are tandem dyes that include the subject water-soluble dipyrromethene-based dyes, e.g., as donor chromophores. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US11702547B2
公开(公告)日：2023-07-18
申请号：US17531067
申请日：2021-11-19
发明人： Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
CPC分类号： C09B69/109 , G01N33/582
摘要： Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20200002540A1
公开(公告)日：2020-01-02
申请号：US16368513
申请日：2019-03-28
发明人： Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
摘要： Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
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7.发明公开公开(公告)号：US20230324374A1
公开(公告)日：2023-10-12
申请号：US18127568
申请日：2023-03-28
发明人： Glenn Bartholomew , Brian Wall , David Moureau
IPC分类号： G01N33/542 , G01N33/533 , G01N33/543
CPC分类号： G01N33/542 , G01N33/533 , G01N33/54326
摘要： Tandem dyes having internally positioned sensors are provided. Tandem dyes of embodiments of the invention include a peptidic scaffold comprising: one or more donor-fluor amino acid residues each conjugated to a pendant donor fluorophore; one or more acceptor-fluor amino acid residues each conjugated to a pendant acceptor fluorophore; and an internal amino acid residue conjugated to a sensor, e.g., a specific binding member, e.g., for a target analyte. In tandem dyes of embodiments of the invention, the donor and acceptor fluorophores of the are in energy transfer relationship. Also provided are methods of making and using the tandem dyes, as well as kits that include the dyes that find use in embodiments of the methods.
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公开(公告)号：US20230295441A1
公开(公告)日：2023-09-21
申请号：US18201649
申请日：2023-05-24
发明人： Glenn Bartholomew , Yongchao Liang , Brian Wall , David Moureau
CPC分类号： C09B69/109 , G01N33/582
摘要： Water soluble light harvesting multichromophores having pendant chromophore groups are provided. The light harvesting multichromophore has a polymeric backbone including non-conjugated repeat units and a plurality of pendant donor chromophore groups linked to a non-conjugated repeat unit of the polymeric backbone. A pendant chromophore group can be a BODIPY group substituted with one or more water soluble groups. Polymeric tandem dyes based on the subject multichromophores are provided that further include an acceptor fluorophore linked to a non-conjugated repeat unit of the polymeric backbone and configured in energy-receiving proximity to a pendant donor chromophore group. Also provided are labelled specific binding members that include the subject polymeric tandem dyes. Methods of evaluating a sample for a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are provided. Systems and kits for practicing the subject methods are also provided.
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公开(公告)号：US20230295440A1
公开(公告)日：2023-09-21
申请号：US18120554
申请日：2023-03-13
IPC分类号： C09B69/10 , G01N33/58 , G01N33/563 , G01N33/68 , G01N33/543
CPC分类号： C09B69/109 , G01N33/582 , G01N33/563 , G01N33/68 , G01N33/54326 , G01N2446/90
摘要： Violet excitable tandem dyes are provided. Tandem dyes of embodiments of the invention include: a non-conjugated polymeric backbone; one or more violet excitable donor fluorophores linked to the non-conjugated polymeric backbone; and one or more acceptor fluorophores linked to the non-conjugated polymeric backbone; where the donor and acceptor fluorophores are in energy transfer relationship. Also provided are methods of making and using the tandem dyes, as well as kits that include the dyes and find use in embodiments of the methods.
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公开(公告)号：US20230152327A1
公开(公告)日：2023-05-18
申请号：US17876349
申请日：2022-07-28
CPC分类号： G01N33/582 , C09B23/0075
摘要： Water-soluble dipyrromethene-based dyes are provided herein. In certain cases, the water-soluble dye is a dipyrromethene-boron dye. Embodiments of the subject water-soluble dipyrromethene-based dyes have a narrow absorption maxima in the yellow green range close to or at the 561 nm laser line, and are suitable for use in tandem dye systems. Embodiments of the subject dyes efficiently transfer energy to an acceptor chromophore while a second dipyrromethene-based dye of the same or different chemical structure is within energy-receiving proximity therewith. Also provided herein are tandem dyes that include the subject water-soluble dipyrromethene-based dyes, e.g., as donor chromophores. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.
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