公开(公告)号：US09932606B2

公开(公告)日：2018-04-03

申请号：US15098342

申请日：2016-04-14

Applicant: Beijing Bio-Targeting Therapeutics Technology Inc.

Inventor： Yaohe Wang ,  Guozhong Jiang ,  Hanshi Wong ,  Fengyu Cao ,  Nick Lemoine

IPC: C12N15/64 ,  C12N15/86 ,  A61K48/00 ,  A61P35/00 ,  A61K35/761 ,  C12N7/00

CPC classification number: C12N15/86 ,  A61K35/761 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343

Abstract: A method for reconstructing a replication-selective oncolytic adenovirus using a subgroup B recombinant human adenovirus vector Ad11-5EP. The method includes: 1) deleting E1A CR2 gene and/or anti-apoptotic gene E1B 21K that is necessary for viability of an adenovirus in normal cells but not necessary in tumor cells; 2) inserting a tumor-specific promoter to drive the expression of E1A gene; 3) re-directing a cellular tropism of Ad11-5EP according to receptors on a tumor cell surface; or 4) allowing adenovirus to selectively replicate in tumor cells.

公开(公告)号：US10294493B2

公开(公告)日：2019-05-21

申请号：US15904408

申请日：2018-02-25

Applicant: Beijing Bio-Targeting Therapeutics Technology Inc.

Inventor： Yaohe Wang ,  Guozhong Jiang ,  Hanshi Wong ,  Fengyu Cao ,  Nick Lemoine

IPC: A61K35/761 ,  C12N15/86 ,  C12N15/861 ,  C12N7/00

Abstract: A subgroup B recombinant human adenovirus vector Ad11-5EP (SEQ ID NO: 1), which is constructed by a method including: substituting a 365 bp fragment containing an enhancer and a promoter of an upstream coding sequence of Ad5 E1A (SEQ ID NO: 2) for a corresponding region of a serotype Ad11 (SEQ ID NO: 3) of a subgroup B human adenovirus vector by homologous recombination to construct the subgroup B recombinant human adenovirus vector Ad11-5EP (SEQ ID NO: 1).

公开(公告)号：US09315827B2

公开(公告)日：2016-04-19

申请号：US14093078

申请日：2013-11-29

Applicant: Beijing Bio-Targeting Therapeutics Technology Inc.

Inventor： Yaohe Wang ,  Guozhong Jiang ,  Hanshi Wong ,  Fengyu Cao ,  Nick Lemoine

IPC: C12N15/64 ,  C12N15/66 ,  C12N15/86 ,  A61K35/761

CPC classification number: C12N15/86 ,  A61K35/761 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343

Abstract: A method for constructing a subgroup B recombinant human adenovirus vector Ad11-5EP. The method includes substituting a 365 bp fragment including an enhancer and a promoter of an upstream coding sequence of Ad5 E1A for a corresponding region of a serotype Ad11 of the subgroup B human adenovirus vector by homologous recombination to construct the subgroup B recombinant human adenovirus vector Ad11-5EP. A subgroup B recombinant human adenovirus vector Ad11-5EP constructed by the method and the use thereof for treatment of tumors are also provided.

Abstract translation: 一种构建亚组B重组人腺病毒载体Ad11-5EP的方法。 该方法包括通过同源重组将包含增强子的365bp片段和Ad5 E1A上游编码序列的启动子替换为亚组B人腺病毒载体的血清型Ad11的相应区域，以构建亚组B重组人腺病毒载体Ad11 -5EP。 还提供了通过该方法构建的亚组B重组人腺病毒载体Ad11-5EP及其用于治疗肿瘤的用途。