公开(公告)号：US20120270234A1

公开(公告)日：2012-10-25

申请号：US12987863

申请日：2011-01-10

申请人： Beom-Seok YANG ,  Sung-Dae Park

发明人： Beom-Seok YANG ,  Sung-Dae Park

IPC分类号： C12N9/12 ,  C12Q1/48 ,  G01N21/76 ,  C12N15/866

CPC分类号： C07K14/705 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/23 ,  C07K2319/35 ,  C12N9/1205

摘要： The present invention relates to a protein containing a modified DDR (Discoidin Domain Receptor) 2 cytosolic tyrosine kinase domain having an increased autophosphorylation and tyrosine kinase activity; a method for preparing a large amount of a protein containing DDR2 cytosolic tyrosine kinase domain, having an increased autophosphorylation and tyrosine kinase activity by inducing phosphorylations of tyrosines by a co-expression with Src or Src related proteins in host cells, or by H2O2 processing of host cells, or a site directed mutation modifying at least one of tyrosines to other amino acid; and a use thereof as a target material in developing medical drugs for treating a disease caused by an excessively activated DDR2 autophosphorylation and tyrosine kinase activity.

摘要翻译： 本发明涉及含有增加的自磷酸化和酪氨酸激酶活性的修饰的DDR（盘带蛋白结构域受体）2胞质酪氨酸激酶结构域的蛋白质; 通过与宿主细胞中的Src或Src相关蛋白的共表达，或通过H 2 O 2处理，通过诱导酪氨酸的磷酸化，具有增加的自磷酸化和酪氨酸激酶活性的DDR2胞质酪氨酸激酶结构域的蛋白质的制备方法 宿主细胞或将至少一种酪氨酸修饰至其它氨基酸的位点定向突变; 以及其用作开发用于治疗由过度活化的DDR2自磷酸化和酪氨酸激酶活性引起的疾病的药物的目标材料。