公开(公告)号：US20120171738A1

公开(公告)日：2012-07-05

申请号：US13496342

申请日：2010-09-16

申请人： Bernhard Hauer ,  Cecilia Kvarnström Branneby ,  Karl Hult ,  Anders Mangnusson ,  Anders Hamberg

发明人： Bernhard Hauer ,  Cecilia Kvarnström Branneby ,  Karl Hult ,  Anders Mangnusson ,  Anders Hamberg

IPC分类号： C12P7/62 ,  C12N1/21 ,  C12N15/63 ,  C12N9/20 ,  C12N15/56

CPC分类号： C12P7/62 ,  C12N9/20 ,  C12P41/004

摘要： The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacyl-glycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.

摘要翻译： 本发明涉及由三酰基甘油脂肪酶突变体催化的单酰化多元醇的生物催化方法，例如衍生自南极假丝酵母脂肪酶B（CALB）; 通过相同的酶突变体催化的对映选择性制备不对称单酰化多元醇的生物催化方法; 以及在制备单酰化多元醇的方法中使用突变的三酰基甘油脂肪酶。 本发明还提供了新的突变体，其编码序列和携带所述编码序列的重组微生物。

公开(公告)号：US08715970B2

公开(公告)日：2014-05-06

申请号：US13496342

申请日：2010-09-16

申请人： Bernhard Hauer ,  Cecilia Kvarnström Branneby ,  Karl Hult ,  Anders Magnusson ,  Anders Hamberg

发明人： Bernhard Hauer ,  Cecilia Kvarnström Branneby ,  Karl Hult ,  Anders Magnusson ,  Anders Hamberg

IPC分类号： C12P7/62 ,  C12N1/21

CPC分类号： C12P7/62 ,  C12N9/20 ,  C12P41/004

摘要： The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.

摘要翻译： 本发明涉及由三酰基甘油脂肪酶突变体催化的单酰化多元醇的生物催化方法，例如衍生自南极假丝酵母脂肪酶B（CALB）; 通过相同的酶突变体催化的对映选择性制备不对称单酰化多元醇的生物催化方法; 以及在制备单酰化多元醇的方法中使用突变的三酰基甘油脂肪酶。 本发明还提供了新的突变体，其编码序列和携带所述编码序列的重组微生物。

公开(公告)号：US20140242651A1

公开(公告)日：2014-08-28

申请号：US14220301

申请日：2014-03-20

申请人： Bernhard Hauer ,  Cecilia Kvarnström Branneby ,  Karl Hult ,  Anders Magnusson ,  Anders Hamberg

发明人： Bernhard Hauer ,  Cecilia Kvarnström Branneby ,  Karl Hult ,  Anders Magnusson ,  Anders Hamberg

IPC分类号： C12P7/62 ,  C12N9/20

CPC分类号： C12P7/62 ,  C12N9/20 ,  C12P41/004

摘要： The present invention relates to a biocatalytic method of preparing a mono-acylated polyol catalyzed by triacylglycerol lipase mutants, as for example derived from Candida antarctica lipase B (CALB); a biocatalytic method of enantioselectively preparing an asymmetric mono-acylated polyol, catalyzed by the same enzyme mutants; as well as the use of a mutated triacylglycerol lipase in a method of preparing mono-acylated polyols. The invention also provides novel mutants, coding sequences thereof, and recombinant microorganisms carrying said coding sequences.

摘要翻译： 本发明涉及由三酰基甘油脂肪酶突变体催化的单酰化多元醇的生物催化方法，例如衍生自南极假丝酵母脂肪酶B（CALB）; 通过相同的酶突变体催化的对映选择性制备不对称单酰化多元醇的生物催化方法; 以及在制备单酰化多元醇的方法中使用突变的三酰基甘油脂肪酶。 本发明还提供了新的突变体，其编码序列和携带所述编码序列的重组微生物。