公开(公告)号：US20140112936A1

公开(公告)日：2014-04-24

申请号：US13884823

申请日：2011-11-09

申请人： Bing Chen ,  Gary H. Frey ,  Jia Chen

发明人： Bing Chen ,  Gary H. Frey ,  Jia Chen

IPC分类号： A61K39/21 ,  G01N33/569

摘要： Isolated, antigenic polypeptides including a pre-hairpin intermediate conformation of gp41 and vectors encoding such polypeptides are provided. Antibodies that bind to a pre-hairpin intermediate conformation of gp41 and methods of making antibodies a that bind to pre-hairpin intermediate conformation of gp41 are also provided. Vaccines against a pre-hairpin Fd intermediate conformation of gp41, as well as methods of treating subjects infected with HIV, preventing HIV infection, and inhibiting HIV-mediated activities are also provided. Methods of screening compounds that bind to an isolated, pre-hairpin intermediate conformation of gp41 are further provided.

摘要翻译： 提供分离的抗原多肽，包括gp41的前发夹中间体构象和编码这种多肽的载体。 还提供了结合gp41的前发夹中间构象的抗体和制备抗体a的方法，其结合gp41的前发夹中间构象。 还提供了针对gp41的预发夹Fd中间构象的疫苗，以及治疗感染HIV的受试者，预防HIV感染和抑制HIV介导的活性的方法。 还提供了筛选结合gp41的分离的前发夹中间构象的化合物的方法。

公开(公告)号：US10675346B2

公开(公告)日：2020-06-09

申请号：US13884823

申请日：2011-11-09

申请人： Bing Chen ,  Gary H. Frey ,  Jia Chen

发明人： Bing Chen ,  Gary H. Frey ,  Jia Chen

IPC分类号： A61K39/21 ,  C07K14/005 ,  G01N33/569 ,  A61K39/00

摘要： Isolated, antigenic polypeptides including a pre-hairpin intermediate conformation of gp41 and vectors encoding such polypeptides are provided. Exemplary pre-hairpin intermediate conformations of gp41 include an oligomerization domain; a heptad repeat 2 motif; and a membrane-proximal external region, where the polypeptide lacks a heptad repeat 1 motif, and where the isolated, antigenic polypeptides elicit production of a broadly neutralizing antibody against HIV when injected into a subject. Antibodies that bind to a pre-hairpin intermediate conformation of gp41 and methods of making antibodies a that bind to pre-hairpin intermediate conformation of gp41 are also provided. Vaccines against a pre-hairpin intermediate conformation of gp41, as well as methods of treating subjects infected with HIV, preventing HIV infection, and inhibiting HIV-mediated activities are also provided. Methods of screening compounds that bind to an isolated, pre-hairpin intermediate conformation of gp41 are further provided.

公开(公告)号：US08741310B2

公开(公告)日：2014-06-03

申请号：US12869967

申请日：2010-08-27

申请人： Bing Chen ,  Gary H. Frey ,  Stephen C. Harrison

发明人： Bing Chen ,  Gary H. Frey ,  Stephen C. Harrison

IPC分类号： A61K39/21

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  C12N2710/14143 ,  C12N2740/16122 ,  C12N2740/16134

摘要： Isolated, antigenic polypeptides including a prehairpin intermediate conformation of gp41 and vectors encoding such polypeptides are provided. Antibodies that bind to a prehairpin intermediate conformation of gp41 and methods of making antibodies a that bind to prehairpin intermediate conformation of gp41 are also provided. Vaccines against a prehairpin intermediate conformation of gp41, as well as methods of treating subjects infected with HIV, preventing HIV infection, and inhibiting HIV-mediated activities are also provided. Methods of screening compounds that bind to an isolated, prehairpin intermediate conformation of gp41 are further provided.

摘要翻译： 提供了分离的抗原多肽，包括gp41的前体前体中间体构象和编码这些多肽的载体。 还提供了结合gp41的前凝胶中间体构象的抗体和制备抗体a的方法，其与gp41的前凝胶中间构象结合。 还提供了针对gp41的前凝胶中间构象的疫苗以及治疗HIV感染者，预防HIV感染和抑制HIV介导活性的方法。 还提供了筛选结合gp41的分离的前贴片中间构象的化合物的方法。

公开(公告)号：US20110086041A1

公开(公告)日：2011-04-14

申请号：US12869967

申请日：2010-08-27

申请人： Bing Chen ,  Gary H. Frey ,  Stephen C. Harrison

发明人： Bing Chen ,  Gary H. Frey ,  Stephen C. Harrison

IPC分类号： A61K39/42 ,  C07K14/16 ,  C12N15/63 ,  A61K39/00 ,  A61P31/18 ,  G01N33/53 ,  C40B30/04 ,  C07K16/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  C12N2710/14143 ,  C12N2740/16122 ,  C12N2740/16134

摘要： Isolated, antigenic polypeptides including a prehairpin intermediate conformation of gp41 and vectors encoding such polypeptides are provided. Antibodies that bind to a prehairpin intermediate conformation of gp41 and methods of making antibodies a that bind to prehairpin intermediate conformation of gp41 are also provided. Vaccines against a prehairpin intermediate conformation of gp41, as well as methods of treating subjects infected with HIV, preventing HIV infection, and inhibiting HIV-mediated activities are also provided. Methods of screening compounds that bind to an isolated, prehairpin intermediate conformation of gp41 are further provided.

摘要翻译： 提供了分离的抗原多肽，包括gp41的前体前体中间体构象和编码这些多肽的载体。 还提供了结合gp41的前凝胶中间体构象的抗体和制备抗体a的方法，其与gp41的前凝胶中间构象结合。 还提供了针对gp41的前凝胶中间构象的疫苗以及治疗HIV感染者，预防HIV感染和抑制HIV介导活性的方法。 还提供了筛选结合gp41的分离的前贴片中间构象的化合物的方法。