公开(公告)号：US20150226703A1

公开(公告)日：2015-08-13

申请号：US14577824

申请日：2014-12-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

IPC: G01N27/447 ,  C07K1/26

CPC classification number: G01N27/44747 ,  C07K1/26 ,  Y10S436/826

Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

Abstract translation: 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。

公开(公告)号：US09664646B2

公开(公告)日：2017-05-30

申请号：US14085472

申请日：2013-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jason McKee ,  Cory Panattoni

IPC: G01N27/447 ,  B29C45/16 ,  G01N27/453

CPC classification number: G01N27/44747 ,  G01N27/44756 ,  G01N27/453

Abstract: The detrimental effect of oxygen exposure on the formation of polyacrylamide electrophoresis gels in oxygen-permeable plastic cassettes is reduced or eliminated either by the use of an oxygen barrier material over the surfaces of the plastic walls of the cassette through which oxygen would otherwise pass into the cassette cavity, or by the incorporation of an oxygen scavenger in the plastic from which the cassette walls are made, or both.

公开(公告)号：US09150611B2

公开(公告)日：2015-10-06

申请号：US13940147

申请日：2013-07-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nikolas Chmiel ,  Cory Panattoni

IPC: G01N27/447 ,  C07K1/26

CPC classification number: C07K1/26

Abstract: The present invention relates to an aqueous transfer buffer that provides superior efficiency in transferring polypeptides of a broad range of molecular weight from a matrix used in electrophoresis to another immobilized surface. Also disclosed are electrophoretic methods and devices in which the aqueous transfer solution of this invention is used.

Abstract translation: 本发明涉及一种水性转移缓冲液，其在从电泳中使用的基质转移到另一种固定化表面时提供了将广泛范围分子量的多肽转移到其它固定化表面上的优异效率 还公开了使用本发明的水转移溶液的电泳方法和装置。

公开(公告)号：US09486741B2

公开(公告)日：2016-11-08

申请号：US13934936

申请日：2013-07-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：US20140138248A1

公开(公告)日：2014-05-22

申请号：US14085472

申请日：2013-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jason McKee ,  Cory Panattoni

IPC: G01N27/447 ,  B29C70/88 ,  B29C45/16

CPC classification number: G01N27/44747 ,  G01N27/44756 ,  G01N27/453

Abstract: The detrimental effect of oxygen exposure on the formation of polyacrylamide electrophoresis gels in oxygen-permeable plastic cassettes is reduced or eliminated either by the use of an oxygen barrier material over the surfaces of the plastic walls of the cassette through which oxygen would otherwise pass into the cassette cavity, or by the incorporation of an oxygen scavenger in the plastic from which the cassette walls are made, or both.

Abstract translation: 氧曝露对透氧塑料盒中聚丙烯酰胺电泳凝胶形成的有害影响通过在盒的塑料壁的表面上使用氧气阻挡材料来减少或消除，否则氧会透过 或通过将氧清除剂并入制造盒壁的塑料中，或两者兼有。

公开(公告)号：US10732145B2

公开(公告)日：2020-08-04

申请号：US15758445

申请日：2016-09-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton McKee ,  Cory Panattoni ,  Paul Liu

IPC: G01N27/447 ,  G01N33/68

Abstract: Gel electrophoresis receptacles and methods of using such receptacles are provided. In one embodiment, a receptacle comprises a cavity for receiving an electrophoresis gel, wherein at least a portion of the receptacle is formed from cyclic olefin copolymer or cyclic olefin polymer.

公开(公告)号：US10119934B2

公开(公告)日：2018-11-06

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：US20180252676A1

公开(公告)日：2018-09-06

申请号：US15758445

申请日：2016-09-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Clayton McKee ,  Cory Panattoni ,  Paul Liu

IPC: G01N27/447 ,  G01N33/68

CPC classification number: G01N27/44747 ,  G01N27/44704 ,  G01N27/4473 ,  G01N27/44743 ,  G01N27/44782 ,  G01N33/6803

Abstract: Gel electrophoresis receptacles and methods of using such receptacles are provided. In one embodiment, a receptace comprises a cavity for receiving an electrophoresis gel, wherein at least a portion of the receptacle is formed from cyclic olefin copolymer or cyclic olefin polymer.

公开(公告)号：US20170067852A1

公开(公告)日：2017-03-09

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  C08F220/56 ,  B01D57/02

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。

公开(公告)号：US20140014514A1

公开(公告)日：2014-01-16

申请号：US13940147

申请日：2013-07-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nickolas Chmiel ,  Cory Panattoni

IPC: C07K1/26

CPC classification number: C07K1/26

Abstract: The present invention relates to an aqueous transfer buffer that provides superior efficiency in transferring polypeptides of a broad range of molecular weight from a matrix used in electrophoresis to another immobilized surface. Also disclosed are electrophoretic methods and devices in which the aqueous transfer solution of this invention is used.

Abstract translation: 本发明涉及一种水性转移缓冲液，其在从电泳中使用的基质转移到另一种固定化表面时提供了将广泛范围分子量的多肽转移到其它固定化表面上的优异效率。 还公开了使用本发明的水转移溶液的电泳方法和装置。