METHOD AND COMPOSITION FOR QUANTIFYING PROTEIN USING TAGGED STANDARDS

    公开(公告)号:US20220178874A1

    公开(公告)日:2022-06-09

    申请号:US17544591

    申请日:2021-12-07

    Abstract: Methods and reference compositions for quantifying protein using tagged standards. In an exemplary method, a reference composition and a protein may be electrophoresed in respective lanes of a gel. The reference composition may include quantitation standards of different size and each including a tag present at a different concentration. The quantitation standards and the protein may be transferred from the gel to a solid support to create a blot. Luminescence may be detected from the blot to obtain respective luminescence values separately representing an abundance of the tag of each quantitation standard and an abundance of the protein. A quantity of the protein may be determined using the respective luminescence values.

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