公开(公告)号：US20140141467A1

公开(公告)日：2014-05-22

申请号：US14162382

申请日：2014-01-23

申请人： Biocon Limited

发明人： Sanjay Tiwari ,  Mukesh Babuappa Patale ,  Saurabh Garg ,  Mayank Kumar Garg ,  Sulekha Joshi ,  Chittnalli Ramegowda Naveen Kumar ,  Bimal Kumar ,  Anuj Goel ,  Harish Iyer

IPC分类号： C12P21/00 ,  C12N9/52 ,  C07K14/535 ,  C12N9/20

CPC分类号： C12P21/00 ,  C07K14/535 ,  C12N9/20 ,  C12N9/52 ,  C12P21/02

摘要： The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

摘要翻译： 本发明证实了碳酸酰胺如尿素或其衍生物，氨基甲酸酯，碳二亚胺和硫代氨基甲酰胺作为含氮补充剂在发酵培养基中用于生产重组蛋白以实现增强的生物转化速率和肽如胰岛素和胰岛素类似物，毒蜥外泌肽和酶的效用 作为使用甲醇诱导型真菌表达系统如毕赤酵母的脂肪酶。

公开(公告)号：US09255285B2

公开(公告)日：2016-02-09

申请号：US14162382

申请日：2014-01-23

申请人： BIOCON LIMITED

发明人： Sanjay Tiwari ,  Mukesh Babuappa Patale ,  Saurabh Garg ,  Mayank Kumar Garg ,  Sulekha Joshi ,  Chittnalli Ramegowda Naveen Kumar ,  Bimal Kumar ,  Anuj Goel ,  Harish Iyer

IPC分类号： C12N1/00 ,  C12P21/04 ,  C12N9/00 ,  C12P21/00 ,  C07K14/535 ,  C12N9/20 ,  C12N9/52 ,  C12P21/02

CPC分类号： C12P21/00 ,  C07K14/535 ,  C12N9/20 ,  C12N9/52 ,  C12P21/02

摘要： The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogs, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

摘要翻译： 本发明证明了碳酸酰胺如尿素或其衍生物，氨基甲酸酯，碳二亚胺和硫代氨基甲酰胺作为含氮补充剂在发酵培养基中用于生产重组蛋白以实现增强的生物转化速率和肽如胰岛素和胰岛素类似物，毒蜥外泌肽和酶的效用 作为使用甲醇诱导型真菌表达系统如毕赤酵母的脂肪酶。