-
公开(公告)号：US20120202218A1
公开(公告)日：2012-08-09
申请号：US13063610
申请日：2009-09-14
申请人： Bo Liedberg , Daniel Aili , Lars Baltzer , Karin Enander , Johan Rydberg
发明人： Bo Liedberg , Daniel Aili , Lars Baltzer , Karin Enander , Johan Rydberg
IPC分类号： G01N33/573 , C40B60/12 , G01N33/566 , B82Y15/00
CPC分类号： G01N33/54313 , B82Y15/00 , B82Y30/00 , G01N33/54306
摘要： A method for determining the presence of a compound in a liquid solution, by admixing the liquid solution with a plurality of nanoparticles; providing conditions effective to cause aggregation of the nanoparticles in the liquid solution in the absence of said compound in the liquid solution; and observing a detectable signal reflecting the amount of aggregation of nanoparticles in the liquid solution, wherein the presence of the compound in the liquid solution results in a detectable signal reflecting a reduced amount of aggregation of nanoparticles in the liquid solution, in comparison to the amount of aggregation of nanoparticles obtained in the liquid solution in the absence of the compound therein. A nanoparticle, a composition, a kit and a for multi-well plate for use in the method are also disclosed. In some embodiments the association is cation-, anion and/or PH induced e.g. by using helix-loop-helix polypeptides as first and second molecules attached to the nanoparticles. The first molecules are directed to the target compound, the second molecules allow for aggregation of the nanoparticles.
摘要翻译： 一种通过将液体溶液与多种纳米颗粒混合来确定化合物在液体溶液中的存在的方法; 提供在液体溶液中不存在所述化合物的情况下有效引起纳米颗粒在液体溶液中的聚集的条件; 并观察反映液体溶液中纳米颗粒聚集量的可检测信号，其中化合物在液体溶液中的存在导致反映在液体溶液中纳米颗粒聚集量减少的可检测信号， 在不存在化合物的情况下在液体溶液中获得的纳米颗粒的聚集。 还公开了用于该方法的纳米颗粒，组合物，试剂盒和用于多孔板的试剂盒。 在一些实施方案中，所述缔合是阳离子，阴离子和/或PH诱导的。 通过使用螺旋 - 环 - 螺旋多肽作为连接到纳米颗粒的第一和第二分子。 第一分子被引导到目标化合物，第二分子允许纳米颗粒的聚集。
-
公开(公告)号：US07569661B2
公开(公告)日：2009-08-04
申请号：US10512295
申请日：2003-03-26
申请人： Lars Baltzer , Gunnar Dolphin , Bo Liedberg , Ingemar Lundström
发明人： Lars Baltzer , Gunnar Dolphin , Bo Liedberg , Ingemar Lundström
IPC分类号： A61K38/00
CPC分类号： B82Y30/00 , C07K1/047 , C07K1/1077 , C07K14/001 , C07K17/00
摘要： Novel peptide having a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 are disclosed and also polypeptide scaffold consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, said helix-loop-helix motifs having sequences according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 which may comprise a fluorescent probe at the side chain of Lys15 and a ligand with affinity for a target molecule at the side chain of Lys8 or Lys34.Also disclosed are polypeptide scaffolds for use in biosensing applications with or without the polypeptide scaffold anchored to a solid surface.
摘要翻译： 具有SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 公开了3号，并且还包括由两个二聚的螺旋 - 环 - 螺旋基序形成的四个螺旋束组成的多肽支架，所述螺旋 - 环 - 螺旋基序具有根据SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 可以包含Lys15侧链上的荧光探针和对Lys8或Lys34侧链上的靶分子具有亲和力的配体。 还公开了用于生物感测应用的多肽支架，其具有或不具有固定到固体表面的多肽支架。
-
公开(公告)号：US20100099575A1
公开(公告)日：2010-04-22
申请号：US12533636
申请日：2009-07-31
申请人： Lars Baltzer , Gunnar Dolphin , Bo Liedberg , Ingemar Lundstrom
发明人： Lars Baltzer , Gunnar Dolphin , Bo Liedberg , Ingemar Lundstrom
CPC分类号： B82Y30/00 , C07K1/047 , C07K1/1077 , C07K14/001 , C07K17/00
摘要： Novel peptide having a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 are disclosed and also polypeptide scaffold consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, said helix-loop-helix motifs having sequences according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 which may comprise a fluorescent probe at the side chain of Lys15 and a ligand with affinity for a target molecule at the side chain of Lys8 or Lys34.Also disclosed are polypeptide scaffolds for use in biosensing applications with or without the polypeptide scaffold anchored to a solid surface.
摘要翻译： 具有SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 公开了3号，并且还包括由两个二聚的螺旋 - 环 - 螺旋基序形成的四个螺旋束组成的多肽支架，所述螺旋 - 环 - 螺旋基序具有根据SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 可以包含Lys15侧链上的荧光探针和对Lys8或Lys34侧链上的靶分子具有亲和力的配体。 还公开了用于生物感测应用的多肽支架，其具有或不具有固定到固体表面的多肽支架。
-
公开(公告)号：US20050245727A1
公开(公告)日：2005-11-03
申请号：US10512295
申请日：2003-03-26
申请人： Lars Baltzer , Gunnar Dolphin , Bo Liedberg , Ingemar Lundstrom
发明人： Lars Baltzer , Gunnar Dolphin , Bo Liedberg , Ingemar Lundstrom
CPC分类号： B82Y30/00 , C07K1/047 , C07K1/1077 , C07K14/001 , C07K17/00
摘要： Novel peptide having a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 are disclosed and also polypeptide scaffold consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, said helix-loop-helix motifs having sequences according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 which may comprise a fluorescent probe at the side chain of Lys15 and a ligand with affinity for a target molecule at the side chain of Lys8 or Lys34. Also disclosed are polypeptide scaffolds for use in biosensing applications with or without the polypeptide scaffold anchored to a solid surface.
摘要翻译： 具有SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 公开了3号，并且还包括由两个二聚的螺旋 - 环 - 螺旋基序形成的四个螺旋束组成的多肽支架，所述螺旋 - 环 - 螺旋基序具有根据SEQ ID NO： ID。 第1号，SEQ。 ID。 2号和/或SEQ。 ID。 可以包含Lys15侧链上的荧光探针和对Lys8或Lys34侧链上的靶分子具有亲和力的配体。 还公开了用于生物感测应用的多肽支架，其具有或不具有固定到固体表面的多肽支架。
-
公开(公告)号：US20060057635A1
公开(公告)日：2006-03-16
申请号：US10517320
申请日：2003-06-18
申请人： Per Mansson , Bo Liedberg
发明人： Per Mansson , Bo Liedberg
CPC分类号： B82Y30/00 , B82Y15/00 , G01N33/54353 , G01N2610/00 , Y10T428/31678
摘要： A coated metal surface on a solid support, wherein the coating consists of a self-assembled monolayer (SAM) of oligo(ethylene glycol)-terminated amide group-containing alkyl thiols firmly attached to the metal surface via the thiol-end and low molecular weight antigens bound via an amide-group to the SAM-forming OEG molecule, wherein the alkyl portion has 1-20 methylene groups, wherein the oligo(ethylene glycol) portion has 1-15 ethylene oxy units, and wherein the antigens, such as explosives and narcotics, are optionally reversibly bound to antibodies specific for the antigens, is disclosed. The coated metal surface on a solid support may be used in a method of detecting analyte antigens as part of an analysis device, such as a Piezoelectric Crystal Microbalance device or a Surface Plasmon Resonance biosensor, for detection in an aqueous solution of an analyte antigen with higher affinity to an antibody than the antigen of the coating by monitoring the displacement of the antibody from the coating.
-
公开(公告)号：US20090168068A1
公开(公告)日：2009-07-02
申请号：US12289218
申请日：2008-10-23
申请人： Per Mansson , Bo Liedberg
发明人： Per Mansson , Bo Liedberg
IPC分类号： G01N21/55 , G01N33/553 , B05D1/36 , B32B15/04
CPC分类号： B82Y30/00 , B82Y15/00 , G01N33/54353 , G01N2610/00 , Y10T428/31678
摘要： A coated metal surface on a solid support, wherein the coating consists of a self-assembled monolayer (SAM) of oligo(ethylene glycol)-terminated amide group-containing alkyl thiols firmly attached to the metal surface via the thiol-end and low molecular weight antigens bound via an amide-group to the SAM-forming OEG molecule, wherein the alkyl portion has 1-20 methylene groups, wherein the oligo(ethylene glycol) portion has 1-15 ethylene oxy units, and wherein the antigens, such as explosives and narcotics, are optionally reversibly bound to antibodies specific for the antigens, is disclosed. The coated metal surface on a solid support may be used in a method of detecting analyte antigens as part of an analysis device, such as a Piezoelectric Crystal Microbalance device or a Surface Plasmon Resonance biosensor, for detection in an aqueous solution of an analyte antigen with higher affinity to an antibody than the antigen of the coating by monitoring the displacement of the antibody from the coating.
摘要翻译： 固体载体上的涂覆的金属表面，其中涂层由通过硫醇末端和低分子量牢固地连接到金属表面的低聚（乙二醇）封端的含酰胺基的烷基硫醇的自组装单层（SAM）组成 通过酰胺基与SAM形成的OEG分子结合的重量抗原，其中所述烷基部分具有1-20个亚甲基，其中所述低聚（乙二醇）部分具有1-15个乙烯氧基单元，并且其中所述抗原，例如 爆炸物和麻醉剂任选地可逆地结合抗原特异性抗体。 固体支持物上的涂覆的金属表面可以用于检测作为分析装置的一部分的分析物抗原的方法，例如压电晶体微量天平装置或表面等离子体共振生物传感器，用于在分析物抗原的水溶液中检测， 通过监测来自涂层的抗体的置换，对抗体比涂层的抗原更高的亲和性。
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.045 秒)
