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    Production of GGL with low environmental impact
    1.
    发明申请
    Production of GGL with low environmental impact 有权
    GGL生产环境影响低

    公开(公告)号：US20060134742A1

    公开(公告)日：2006-06-22

    申请号：US11015563

    申请日：2004-12-17

    申请人： Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula Hicks ,  Holly Jessen ,  Sara McFarlan ,  Willy Obyn ,  Fernando Sanchez-Riera ,  William Schroeder

    发明人： Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula Hicks ,  Holly Jessen ,  Sara McFarlan ,  Willy Obyn ,  Fernando Sanchez-Riera ,  William Schroeder

    IPC分类号： C12P21/06 ,  C12N9/02 ,  C12N1/21

    CPC分类号： C12P7/58 ,  C12P17/181 ,  C12P19/02

    摘要： A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-γ-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

    摘要翻译： 公开了一种增加肌醇加氧酶比活性的方法。 该方法包括在有效提高肌醇加氧酶比活性的条件下，将包含肌醇加氧酶和不含硫的还原剂的混合物进行温育。 还公开了生产D-葡萄糖醛酸和葡糖苷酸-γ-内酯的方法，包括在有效地将每升混合物形成5克D-葡萄糖醛酸的条件下孵育包括肌醇，肌醇加氧酶和氧气的混合物至400 克D-葡萄糖醛酸每升混合物。 葡糖醛酸-γ-内酯可以由D-葡萄糖醛酸产物制备。 还公开了适用于这种方法的生物体和核酸。