公开(公告)号：US20060134742A1

公开(公告)日：2006-06-22

申请号：US11015563

申请日：2004-12-17

Applicant: Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula Hicks ,  Holly Jessen ,  Sara McFarlan ,  Willy Obyn ,  Fernando Sanchez-Riera ,  William Schroeder

Inventor： Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula Hicks ,  Holly Jessen ,  Sara McFarlan ,  Willy Obyn ,  Fernando Sanchez-Riera ,  William Schroeder

IPC: C12P21/06 ,  C12N9/02 ,  C12N1/21

CPC classification number: C12P7/58 ,  C12P17/181 ,  C12P19/02

Abstract: A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-γ-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

Abstract translation: 公开了一种增加肌醇加氧酶比活性的方法。 该方法包括在有效提高肌醇加氧酶比活性的条件下，将包含肌醇加氧酶和不含硫的还原剂的混合物进行温育。 还公开了生产D-葡萄糖醛酸和葡糖苷酸-γ-内酯的方法，包括在有效地将每升混合物形成5克D-葡萄糖醛酸的条件下孵育包括肌醇，肌醇加氧酶和氧气的混合物至400 克D-葡萄糖醛酸每升混合物。 葡糖醛酸-γ-内酯可以由D-葡萄糖醛酸产物制备。 还公开了适用于这种方法的生物体和核酸。

公开(公告)号：US08551742B2

公开(公告)日：2013-10-08

申请号：US12595817

申请日：2007-04-11

Applicant: Brian Brazeau ,  Jin-Sook Chang ,  Kwang Myung Cho ,  Young Wook Cho ,  Mervyn Desouza ,  Holly J. Jessen ,  So-Young Kim ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Yong-Uk Shin ,  Hyewon Um

Inventor： Brian Brazeau ,  Jin-Sook Chang ,  Kwang Myung Cho ,  Young Wook Cho ,  Mervyn Desouza ,  Holly J. Jessen ,  So-Young Kim ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Yong-Uk Shin ,  Hyewon Um

IPC: C12P13/12 ,  C12N1/20

CPC classification number: C12P13/12 ,  C12N9/1029 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12Y203/01046 ,  Y02P20/52

Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.

公开(公告)号：US20110183383A1

公开(公告)日：2011-07-28

申请号：US12595817

申请日：2007-04-11

Applicant: Brian Brazeau ,  Jin-Sook Chang ,  Kwang Myung Cho ,  Young Wook Cho ,  Mervyn Desouza ,  Holly J. Jessen ,  So-Young Kim ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Yong-Uk Shin ,  Hyewon Um

Inventor： Brian Brazeau ,  Jin-Sook Chang ,  Kwang Myung Cho ,  Young Wook Cho ,  Mervyn Desouza ,  Holly J. Jessen ,  So-Young Kim ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Yong-Uk Shin ,  Hyewon Um

IPC: C12P13/12 ,  C12N1/21 ,  C12N9/10

CPC classification number: C12P13/12 ,  C12N9/1029 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12Y203/01046 ,  Y02P20/52

Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.

Abstract translation: 这里描述的是从转基因途径中的内源基因以及提供直接巯基化途径的外源基因产生甲硫氨酸和相关产物的微生物。 公开了可用于甲硫氨酸和SAMe生产的新型基因。

公开(公告)号：US20060252135A1

公开(公告)日：2006-11-09

申请号：US11411229

申请日：2006-04-26

Applicant: Brian Brazeau ,  Ellen Burke ,  Mervyn DeSouza ,  Steven Gort ,  Paula Hicks ,  Sherry Kollmann ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Fernando Sanchez-Riera ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

Inventor： Brian Brazeau ,  Ellen Burke ,  Mervyn DeSouza ,  Steven Gort ,  Paula Hicks ,  Sherry Kollmann ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Fernando Sanchez-Riera ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

IPC: C12N9/10 ,  C12P13/22 ,  C12N1/21

CPC classification number: C12P17/10 ,  C12N9/1096 ,  C12N9/90

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

公开(公告)号：US20120178967A1

公开(公告)日：2012-07-12

申请号：US13375058

申请日：2010-05-29

Applicant: Todd Banner ,  John Bohlmann ,  Brian Brazeau ,  Tzyy-Jan Han ,  Paul Loucks ,  Shannon Shriver ,  Shuang Zhou

Inventor： Todd Banner ,  John Bohlmann ,  Brian Brazeau ,  Tzyy-Jan Han ,  Paul Loucks ,  Shannon Shriver ,  Shuang Zhou

IPC: C07C59/76 ,  B01D3/10 ,  B01D3/14

CPC classification number: C07C51/44 ,  C07C59/185

Abstract: Systems and methods for producing levulinic acid from fungal biomass are disclosed. In one implementation, a method for distilling levulinic acid from a glucosamine-containing feedstock is disclosed that yields a relatively pure (e.g., 90% or greater) levulinic acid product from an otherwise problematic waste stream.

Abstract translation: 公开了从真菌生物质生产乙酰丙酸的系统和方法。 在一个实施方案中，公开了从含葡糖胺的原料中蒸馏乙酰丙酸的方法，其从另外有问题的废物流中产生相对纯的（例如，90％或更多）乙酰丙酸产物。

公开(公告)号：US07923231B2

公开(公告)日：2011-04-12

申请号：US11015563

申请日：2004-12-17

Applicant: Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula M. Hicks ,  Holly Jessen ,  Sara C. McFarlan ,  Willy Obyn ,  Fernando A. Sanchez-Riera ,  William A. Schroeder

Inventor： Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula M. Hicks ,  Holly Jessen ,  Sara C. McFarlan ,  Willy Obyn ,  Fernando A. Sanchez-Riera ,  William A. Schroeder

IPC: C12N1/21 ,  C12N9/02 ,  C12P21/06

CPC classification number: C12P7/58 ,  C12P17/181 ,  C12P19/02

Abstract: A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-γ-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

Abstract translation: 公开了一种增加肌醇加氧酶比活性的方法。 该方法包括在有效提高肌醇加氧酶比活性的条件下，将包含肌醇加氧酶和不含硫的还原剂的混合物进行温育。 还公开了D-葡萄糖醛酸和葡糖苷酸-γ-内酯的制备方法，包括在有效地将每升混合物形成5克D-葡萄糖醛酸的条件下孵育包括肌醇，肌醇加氧酶和氧气的混合物至400 克D-葡萄糖醛酸每升混合物。 葡糖醛酸-γ-内酯可以由D-葡萄糖醛酸产物制备。 还公开了适用于这种方法的生物体和核酸。

公开(公告)号：US20080020434A1

公开(公告)日：2008-01-24

申请号：US11714279

申请日：2007-03-06

Applicant: Brian Brazeau ,  Mervyn De Souza ,  Steven Gort ,  Paula Hicks ,  Sherry Kollmann ,  Jose Laplaza ,  Sara McFarlan ,  Fernando Sanchez-Riera ,  Christopher Solheid

Inventor： Brian Brazeau ,  Mervyn De Souza ,  Steven Gort ,  Paula Hicks ,  Sherry Kollmann ,  Jose Laplaza ,  Sara McFarlan ,  Fernando Sanchez-Riera ,  Christopher Solheid

IPC: C12P13/02 ,  C07H21/04 ,  C12N1/20 ,  C12N9/00 ,  C12P13/22 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90

CPC classification number: C12N9/1096 ,  C12N9/90 ,  C12P17/10

Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Abstract translation: 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。