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    METHODS AND COMPOSITIONS FOR SELECTING AN IMPROVED PLANT
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR SELECTING AN IMPROVED PLANT 审中-公开
    选择改良植物的方法和组合物

    公开(公告)号：US20100299773A1

    公开(公告)日：2010-11-25

    申请号：US12778525

    申请日：2010-05-12

    申请人： Jennifer R. Hicks ,  Brenda A. Lowe ,  Brian J. Martinell ,  John Phillips ,  John P. Tamulonis ,  Edward J. Williams

    发明人： Jennifer R. Hicks ,  Brenda A. Lowe ,  Brian J. Martinell ,  John Phillips ,  John P. Tamulonis ,  Edward J. Williams

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H1/02

    CPC分类号： C12N15/8289 ,  A01H1/04 ,  A01H5/00 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8286

    摘要： The invention is directed, in an embodiment, to a method for producing a transgenic plant comprising: providing a substantially homozygous plant line suitable for transformation; selecting a subline of the plant line having reduced heterogeneity; transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; recovering at least one transgenic event from the transformation step; and selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. The invention is also directed to a method for producing a plant having a desired trait comprising: providing a substantially homozygous plant line; selecting a subline of the plant line having reduced heterogeneity; crossing at least one individual of the selected subline with a donor parent having at least one desired trait to form at least one second individual having the desired trait; and backcrossing the at least one second individual with the at least one individual of the selected subline as a recurrent parent to form at least one progeny plant having the desired trait.

    摘要翻译： 本发明在一个实施方案中涉及生产转基因植物的方法，其包括：提供适于转化的基本上纯合的植物系; 选择具有减小的异质性的植物系的一个子系; 用具有将所需性状赋予至少一种转化植物的转基因构建体从该亚系转化植物材料; 从转化步骤回收至少一个转基因事件; 并且使用该子系的植物作为对照选择表现出期望性状的期望水平的转基因事件。 本发明还涉及一种用于生产具有所需性状的植物的方法，包括：提供基本上纯合的植物品系; 选择具有减小的异质性的植物系的一个子系; 将所选择的亚系的至少一个个体与具有至少一个所需性状的供体亲本交叉以形成具有所需性状的至少一个第二个体; 以及使所述至少一个第二个体与所选择的亚系中的至少一个个体作为轮回亲本回交以形成具有期望性状的至少一个后代植物。

    Methods for Improving Competency of Plant Cells
    8.
    发明申请
    Methods for Improving Competency of Plant Cells 审中-公开
    提高植物细胞能力的方法

    公开(公告)号：US20120156784A1

    公开(公告)日：2012-06-21

    申请号：US13328222

    申请日：2011-12-16

    申请人： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

    发明人： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

    IPC分类号： C12N5/04

    CPC分类号： C12N15/8205

    摘要： The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

    摘要翻译： 本发明提供了用于提高植物细胞对细菌介导转化的能力的方法，其包括在转化之前将植物细胞与有效量的聚乙二醇（PEG）接触一段时间。 存储和维护用于转化和组织培养的有效植物细胞的能力允许通过提供高峰生产时间的灵活性或生产过程中的计划外中断来更有效地规划和执行大规模实验。 这些方法可用于保持植物细胞在各种储存条件下的活力，从而提高其转化和组织培养的能力。

    Methods for improving competency of plant cells
    9.
    发明授权
    Methods for improving competency of plant cells 有权

    公开(公告)号：US10407685B2

    公开(公告)日：2019-09-10

    申请号：US13328222

    申请日：2011-12-16

    申请人： Whitney R. Adams, Jr. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

    发明人： Whitney R. Adams, Jr. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

    IPC分类号： C12N15/82

    摘要： The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.