Methods for improving competency of plant cells

    公开(公告)号:US10407685B2

    公开(公告)日:2019-09-10

    申请号:US13328222

    申请日:2011-12-16

    IPC分类号: C12N15/82

    摘要: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

    Methods for Improving Competency of Plant Cells
    2.
    发明申请
    Methods for Improving Competency of Plant Cells 审中-公开
    提高植物细胞能力的方法

    公开(公告)号:US20120156784A1

    公开(公告)日:2012-06-21

    申请号:US13328222

    申请日:2011-12-16

    IPC分类号: C12N5/04

    CPC分类号: C12N15/8205

    摘要: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

    摘要翻译: 本发明提供了用于提高植物细胞对细菌介导转化的能力的方法,其包括在转化之前将植物细胞与有效量的聚乙二醇(PEG)接触一段时间。 存储和维护用于转化和组织培养的有效植物细胞的能力允许通过提供高峰生产时间的灵活性或生产过程中的计划外中断来更有效地规划和执行大规模实验。 这些方法可用于保持植物细胞在各种储存条件下的活力,从而提高其转化和组织培养的能力。