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公开(公告)号:US10407685B2
公开(公告)日:2019-09-10
申请号:US13328222
申请日:2011-12-16
IPC分类号: C12N15/82
摘要: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号:US20120156784A1
公开(公告)日:2012-06-21
申请号:US13328222
申请日:2011-12-16
IPC分类号: C12N5/04
CPC分类号: C12N15/8205
摘要: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
摘要翻译: 本发明提供了用于提高植物细胞对细菌介导转化的能力的方法,其包括在转化之前将植物细胞与有效量的聚乙二醇(PEG)接触一段时间。 存储和维护用于转化和组织培养的有效植物细胞的能力允许通过提供高峰生产时间的灵活性或生产过程中的计划外中断来更有效地规划和执行大规模实验。 这些方法可用于保持植物细胞在各种储存条件下的活力,从而提高其转化和组织培养的能力。
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公开(公告)号:US20120180166A1
公开(公告)日:2012-07-12
申请号:US13355312
申请日:2012-01-20
申请人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译: 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化,并减少与传统植物组织培养系统相关的劳动力,材料和人体工学成本。
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公开(公告)号:US08513016B2
公开(公告)日:2013-08-20
申请号:US13355312
申请日:2012-01-20
申请人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
IPC分类号: C12N5/00
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
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公开(公告)号:US08581035B2
公开(公告)日:2013-11-12
申请号:US11848579
申请日:2007-08-31
申请人: Jyoti R. Rout
发明人: Jyoti R. Rout
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
摘要翻译: 本发明提供用于鉴定再生转化植物和分化转化植物部分的方法,所述转化植物和分化的转化植物部分在再生细胞以获得分化组织之前不经植物细胞获得选择性条件 在具体实施方案中,植物细胞是玉米植物细胞。 还提供了用于种植和处理植物的方法,包括鉴定显示特定感兴趣特征的植物。
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公开(公告)号:US08395020B2
公开(公告)日:2013-03-12
申请号:US11848648
申请日:2007-08-31
申请人: Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
发明人: Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
摘要翻译: 本发明提供了通过简单且快速的方案转化单子叶植物的方法,以获得能够在4-8周内种植到土壤中的再生植物。 还提供了相关的细胞培养基和生长条件,以及通过该方法获得的植物和植物部分。 此外,还提供了通过本发明的方法筛选用于可变形性的顽固植物基因型的方法。 此外,还提供了通过本发明的方法扩展用于生产转基因植物的优先发展窗口的系统。
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公开(公告)号:US08124411B2
公开(公告)日:2012-02-28
申请号:US11848554
申请日:2007-08-31
申请人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译: 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化,并减少与传统植物组织培养系统相关的劳动力,材料和人体工学成本。
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公开(公告)号:US20080124727A1
公开(公告)日:2008-05-29
申请号:US11848648
申请日:2007-08-31
申请人: Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
发明人: Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
摘要翻译: 本发明提供了通过简单且快速的方案转化单子叶植物的方法,以获得能够在4-8周内种植到土壤中的再生植物。 还提供了相关的细胞培养基和生长条件,以及通过该方法获得的植物和植物部分。 此外,还提供了通过本发明的方法筛选用于可变形性的顽固植物基因型的方法。 此外,还提供了通过本发明的方法扩展用于生产转基因植物的优先发展窗口的系统。
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公开(公告)号:US20080118981A1
公开(公告)日:2008-05-22
申请号:US11848554
申请日:2007-08-31
申请人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
发明人: Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
CPC分类号: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
摘要翻译: 证明了在单个容器中生产稳定转化的玉米植物的方法。 这种方法允许转换过程的自动化,并减少与传统植物组织培养系统相关的劳动力,材料和人体工学成本。
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