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公开(公告)号：US20080276333A1
公开(公告)日：2008-11-06
申请号：US11596037
申请日：2005-06-09
申请人： Brian McGonigle , Johan M. Stoop
发明人： Brian McGonigle , Johan M. Stoop
CPC分类号： C12N15/8247 , C12N15/8216 , C12N15/8218 , C12N15/8245
摘要： Recombinant constructs useful for reducing the expression of at least one target nucleic acid fragment of interest and any substantially similar endogenous nucleic acid fragment of interest are disclosed. In particular, a recombinant construct comprising at least one nucleic acid of interest is inserted between two convergent promoters. A plant or plant organ stably transformed with this construct will have a reduction in expression of the target nucleic acid fragments of interest.
摘要翻译： 公开了可用于降低目标的至少一个靶核酸片段和任何基本上相似的感兴趣的内源性核酸片段的表达的重组构建体。 特别地，将包含至少一种目的核酸的重组构建体插入两个收敛启动子之间。 用该构建体稳定转化的植物或植物器官将具有目标靶核酸片段的表达降低。
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公开(公告)号：US20090100549A1
公开(公告)日：2009-04-16
申请号：US12336578
申请日：2008-12-17
申请人： Johan M. Stoop , Stephen M. Allen , Perry G. Caimi
发明人： Johan M. Stoop , Stephen M. Allen , Perry G. Caimi
CPC分类号： C12N9/1051 , C12N15/8234 , C12N15/8245
摘要： Isolated nucleic acid fragments encoding galactinol synthase are disclosed. Recombinant DNA construct(s) for use in altering expression of endogenous genes encoding galactinol synthase are also disclosed.
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3.发明授权PM29 and LEA3 promoters and use in expression of transgenic genes in plants 有权PM29和LEA3启动子，并用于植物中转基因基因的表达公开(公告)号：US07291765B2
公开(公告)日：2007-11-06
申请号：US11622573
申请日：2007-01-12
申请人： Zhan-Bin Liu , Johan M. Stoop
发明人： Zhan-Bin Liu , Johan M. Stoop
CPC分类号： C12N15/8234 , C12N15/8245
摘要： The promoters of a soybean seed maturation protein PM29 and a soybean late-embryogenesis abundant protein LEA3 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.
摘要翻译： 描述了大豆种子成熟蛋白PM29和大豆晚胚发生丰富蛋白LEA3及其片段的启动子及其在促进植物中一种或多种异源核酸片段表达的用途。
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公开(公告)号：US20120005779A1
公开(公告)日：2012-01-05
申请号：US13071677
申请日：2011-03-25
IPC分类号： C12N15/82 , C12N15/63 , A01H5/10 , A01H5/00 , A23L1/20 , C12Q1/68 , C12N5/10 , C12N1/21 , C12N1/19 , C07H21/04 , C12N9/90
CPC分类号： C12N9/90 , C12N15/8216 , C12N15/8245 , C12N15/8247 , C12N15/8251
摘要： An isolated nucleic acid fragment encoding a plastidic phosphoglucomutase protein is disclosed. Also disclosed is the construction of a chimeric gene encoding all or a substantial portion of the plastidic phosphoglucomutase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the plastidic phosphoglucomutase in a transformed host cell.
摘要翻译： 公开了一种编码质体磷酸果糖调蛋白酶蛋白的分离的核酸片段。 还公开了在正义或反义方向上构建质粒全部或大部分质体磷酸果糖调蛋白酶的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中质体磷酸果糖转化酶水平的改变。
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公开(公告)号：US07253335B2
公开(公告)日：2007-08-07
申请号：US10916247
申请日：2004-08-11
申请人： Stephen M. Allen , Perry G. Caimi , Johan M. Stoop
发明人： Stephen M. Allen , Perry G. Caimi , Johan M. Stoop
CPC分类号： C12N9/1051 , C12N15/8246
摘要： This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
摘要翻译： 本发明涉及编码果糖基转移酶的分离的核酸片段。 更具体地，本发明涉及编码1-FFT，6-SFT或1-SST的多核苷酸。 本发明还涉及在正义或反义方向上构建编码全部或一部分果糖基转移酶的重组DNA构建体，其中重组DNA构建体的表达导致转化宿主细胞中果糖基转移酶水平的变化。
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公开(公告)号：US20120185966A1
公开(公告)日：2012-07-19
申请号：US13435545
申请日：2012-03-30
CPC分类号： C12N9/1051 , C12N15/8246
摘要： This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
摘要翻译： 本发明涉及编码果糖基转移酶的分离的核酸片段。 更具体地，本发明涉及编码1-FFT，6-SFT或1-SST的多核苷酸。 本发明还涉及在正义或反义方向上构建编码全部或一部分果糖基转移酶的重组DNA构建体，其中重组DNA构建体的表达导致转化宿主细胞中果糖基转移酶水平的变化。
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公开(公告)号：US08168862B2
公开(公告)日：2012-05-01
申请号：US13011345
申请日：2011-01-21
CPC分类号： C12N9/1051 , C12N15/8246
摘要： This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
摘要翻译： 本发明涉及编码果糖基转移酶的分离的核酸片段。 更具体地，本发明涉及编码1-FFT，6-SFT或1-SST的多核苷酸。 本发明还涉及在正义或反义方向上构建编码全部或一部分果糖基转移酶的重组DNA构建体，其中重组DNA构建体的表达导致转化宿主细胞中果糖基转移酶水平的变化。
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公开(公告)号：US20090300799A1
公开(公告)日：2009-12-03
申请号：US12536516
申请日：2009-08-06
申请人： Johan M. Stoop , Stephen M. Allen , Perry G. Gaimi
发明人： Johan M. Stoop , Stephen M. Allen , Perry G. Gaimi
IPC分类号： A01H5/00 , C12N15/82 , A01H5/10 , C07H21/00 , C12N15/63 , C12N1/00 , C12N9/88 , C12P21/00 , C12N5/04
CPC分类号： C12N9/1051 , C12N15/8234 , C12N15/8245
摘要： Isolated nucleic acid fragments encoding galactinol synthase are disclosed. Recombinant DNA construct(s) for use in altering expression of endogenous genes encoding galactinol synthase are also disclosed.
摘要翻译： 公开了编码半乳糖醇合成酶的分离的核酸片段。 还公开了用于改变编码半乳糖醇合成酶的内源基因的表达的重组DNA构建体。
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公开(公告)号：US07915486B2
公开(公告)日：2011-03-29
申请号：US11928914
申请日：2007-10-30
IPC分类号： A01H1/00 , C12N15/29 , C07K14/415 , C07H21/04
CPC分类号： C12N9/90 , C12N15/8216 , C12N15/8245 , C12N15/8247 , C12N15/8251
摘要： An isolated nucleic acid fragment encoding a plastidic phosphoglucomutase protein is disclosed. Also disclosed is the construction of a chimeric gene encoding all or a substantial portion of the plastidic phosphoglucomutase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the plastidic phosphoglucomutase in a transformed host cell.
摘要翻译： 公开了一种编码质体磷酸果糖调蛋白酶蛋白的分离的核酸片段。 还公开了在正义或反义方向上构建质粒全部或大部分质体磷酸果糖调蛋白酶的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中质体磷酸果糖转化酶水平的改变。
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公开(公告)号：US07803986B2
公开(公告)日：2010-09-28
申请号：US11780546
申请日：2007-07-20
申请人： Johan M. Stoop
发明人： Johan M. Stoop
CPC分类号： C12N9/1051 , C12N15/8246
摘要： This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
摘要翻译： 本发明涉及编码果糖基转移酶的分离的核酸片段。 更具体地，本发明涉及编码1-FFT，6-SFT或1-SST的多核苷酸。 本发明还涉及在正义或反义方向上构建编码全部或一部分果糖基转移酶的重组DNA构建体，其中重组DNA构建体的表达导致转化宿主细胞中果糖基转移酶水平的变化。
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