公开(公告)号：US20220276140A1

公开(公告)日：2022-09-01

申请号：US17702276

申请日：2022-03-23

申请人： Bruker Daltonics GmbH & Co. KG

发明人： Katrin SPARBIER ,  Beatrix WEGEMANN

IPC分类号： G01N1/40 ,  G01N1/28 ,  C12Q1/04 ,  C12Q1/18 ,  G01N33/68

摘要： The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.