-
1.发明公开公开(公告)号：US20230159977A1
公开(公告)日：2023-05-25
申请号：US18056107
申请日：2022-11-16
发明人： Katrin SPARBIER
CPC分类号： C12Q1/18 , G01N33/6851 , G16B40/10 , G16B20/00
摘要： The invention relates to methods for spectrometric characterization of a test cell substrate. The characterization comprises taxonomic classification and determination of a property of interest of the test cell substrate. The characterization may be based on mass-spectrometric measurement data. The property of interest may be a resistance or susceptibility to a growth-influencing factor. After comparing first spectrometric measurement data of the test cell substrate with a provided reference library, a sub-library is created comprising those reference datasets from the reference library that are classified as allowing a taxonomic classification of the test cell substrate. Second spectrometric measurement data after a second preparation of the test cell substrate under conditions that serve to determine a property of interest of the test cell substrate is compared with the sub-library and allow a reliable determination of the property of interest.
-
2.发明申请公开(公告)号：US20220276140A1
公开(公告)日：2022-09-01
申请号：US17702276
申请日：2022-03-23
发明人： Katrin SPARBIER , Beatrix WEGEMANN
摘要： The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.
-
搜索结果
2 条记录 (耗时 0.012 秒)
