公开(公告)号：US12258613B2

公开(公告)日：2025-03-25

申请号：US16492588

申请日：2018-03-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng ,  Alphonsus Hon-Chung Ng

IPC: C12Q1/6804 ,  B01L3/00 ,  C12N15/10

Abstract: Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.

公开(公告)号：US20230069843A1

公开(公告)日：2023-03-09

申请号：US16492588

申请日：2018-03-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng ,  Alphonsus Hon-Chung Ng

IPC: C12N15/10 ,  B01L3/00

Abstract: Compositions and methods for identifying antigen-specific T cells, including determining paired T cell receptor sequences for a specific antigen, are described. Compositions and methods for identifying neoantigen-specific T cells are also described. Microfluidic devices useful for identifying antigen-specific T cells, and methods of using the same, are also described.

公开(公告)号：US11384327B2

公开(公告)日：2022-07-12

申请号：US16347559

申请日：2017-11-01

Applicant: California Institute of Technology ,  Gwangju Institute of Science and Technology (GIST)

Inventor： Songming Peng ,  James R. Heath ,  William Chour ,  Alphonsus Hon-Chung Ng ,  Sung Yang ,  Jongchan Choi ,  Ji-Chul Hyun

IPC: C12M3/06 ,  B01L3/00 ,  G01N33/569 ,  B01F25/433 ,  B01F33/30

Abstract: Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen-specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.