公开(公告)号：US20170044609A1

公开(公告)日：2017-02-16

申请号：US15336748

申请日：2016-10-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen ,  Colleen A. McHugh

IPC: C12Q1/68 ,  G01N33/50 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/6848

Abstract: Methods of prohibiting Xist-dependent silencing of X chromosome genes include targeting the required Xist silencing complex components including SHARP, SMRT, HDAC3, SAF-A, LBR, and the respective binding sites of SHARP, LBR, and SAF-A on Xist, thereby prohibiting Xist repression, allowing for reactivation of the silenced X chromosome genes.

Abstract translation: 禁止X染色体基因Xist依赖性沉默的方法包括靶向所需的Xist沉默复合物成分，包括SHARP，SMRT，HDAC3，SAF-A，LBR以及Xist上SHARP，LBR和SAF-A的各自的结合位点，从而 禁止Xist镇压，允许重新激活沉默的X染色体基因。

公开(公告)号：US11249071B2

公开(公告)日：2022-02-15

申请号：US15138208

申请日：2016-04-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen ,  Colleen A. McHugh

IPC: G01N33/50

Abstract: Methods of prohibiting Xist-dependent silencing of X chromosome genes include targeting the required Xist silencing complex components including SHARP, SMRT, HDAC3, SAF-A, LBR, and the respective binding sites of SHARP, LBR, and SAF-A on Xist, thereby prohibiting Xist repression, allowing for reactivation of the silenced X chromosome genes.

公开(公告)号：US20160313304A1

公开(公告)日：2016-10-27

申请号：US15138208

申请日：2016-04-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen ,  Colleen A. McHugh

IPC: G01N33/50

CPC classification number: G01N33/5023

Abstract: Methods of prohibiting Xist-dependent silencing of X chromosome genes include targeting the required Xist silencing complex components including SHARP, SMRT, HDAC3, SAF-A, LBR, and the respective binding sites of SHARP, LBR, and SAF-A on Xist, thereby prohibiting Xist repression, allowing for reactivation of the silenced X chromosome genes.

Abstract translation: 禁止X染色体基因Xist依赖性沉默的方法包括靶向所需的Xist沉默复合物成分，包括SHARP，SMRT，HDAC3，SAF-A，LBR，以及Xist上SHARP，LBR和SAF-A的相应结合位点，从而 禁止Xist镇压，允许重新激活沉默的X染色体基因。

公开(公告)号：US11197881B2

公开(公告)日：2021-12-14

申请号：US16345666

申请日：2017-10-27

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mitchell Guttman ,  Chun-Kan Chen

IPC: A61K31/706 ,  A61K31/165 ,  A61K31/405 ,  A61K31/473 ,  C12N15/01

Abstract: A reactivation composition for activating or re-activating expression of a silenced X chromosome gene in a cell includes a non-cytotoxic histone deacetylase (HDAC) inhibitor. The reactivation composition includes the non-cytotoxic HDAC inhibitor and may further include a DNA methylation inhibitor. A method of activating or re-activating expression of a silenced X chromosome gene in a cell includes administering a reactivation composition including a non-cytotoxic HDAC inhibitor. The method of activating or re-activating expression of a silenced X chromosome gene may further includes administering a reactivation composition that includes a non-cytotoxic HDAC inhibitor and an inhibitor of DNA methylation.