公开(公告)号：US20170157607A1

公开(公告)日：2017-06-08

申请号：US15436118

申请日：2017-02-17

Applicant: CANON U.S. LIFE SCIENCES, INC.

Inventor： Kenton C. HASSON ,  Gregory A. DALE

IPC: B01L3/00 ,  G01N21/71 ,  G06T7/00 ,  B01L7/00

CPC classification number: B01L7/525 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/18 ,  B01L2300/1811 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1877 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/71 ,  G01N35/08 ,  G06T7/0012 ,  G06T7/11 ,  G06T2207/10004 ,  G06T2207/30072 ,  Y10T436/115831 ,  Y10T436/117497 ,  C12Q2565/629 ,  C12Q2561/113

Abstract: The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. A method according to one embodiment of the invention may include the steps of: forcing a sample of a solution containing real-time PCR reagents to move though a channel; and while the sample is moving through an analysis region of the channel, performing the steps of: (a) cycling the temperature of the sample until the occurrence of a predetermined event; (b) after performing step (a), causing the sample's temperature to gradually increase from a first temperature to a second temperature; and (c) while the step of gradually increasing the sample's temperature is performed, using an image sensor to monitor emissions from the sample.

公开(公告)号：US20160325280A1

公开(公告)日：2016-11-10

申请号：US15062830

申请日：2016-03-07

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Gregory A. DALE ,  Ivor T. KNIGHT

IPC: B01L3/00 ,  B01F13/00 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01F5/0602 ,  B01F5/0647 ,  B01F13/0059 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/049

Abstract: An assembly for performing micro-fluidic assays includes a micro-fluidic chip with access ports and micro-channels in communication with the access ports and a fluid cartridge having internal, fluid-containable chambers and a nozzle associated with each internal chamber that is configured to be coupled with an access port. Reaction fluids, such as sample material, buffer, and/or reagent, contained within the cartridge are dispensed from the cartridge into the access ports and micro-channels of the micro-fluidic chip. Embodiments of the invention include a cartridge which includes a waste compartment for receiving used DNA and other reaction fluids from the micro-channel at the conclusion of the assay.

Abstract translation: 用于执行微流体测定的组件包括具有进入端口和与进入端口连通的微通道的微流体芯片和具有内部可流体容纳室的流体盒和与每个内部室相关联的喷嘴，其配置为 与访问端口耦合。 包含在药筒内的反应流体，例如样品材料，缓冲液和/或试剂从药筒分配到微流体芯片的进入口和微通道中。 本发明的实施例包括一个盒，其包括在分析结束时从微通道接收使用的DNA和其它反应流体的废物隔室。

公开(公告)号：US20170182497A1

公开(公告)日：2017-06-29

申请号：US15457768

申请日：2017-03-13

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Kenton C. HASSON ,  Gregory A. DALE ,  Hiroshi INOUE

IPC: B01L7/00 ,  B01L3/00

CPC classification number: B01L7/525 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/143 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/18 ,  B01L2300/1811 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1877 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/71 ,  G01N35/08 ,  G06T7/0012 ,  G06T7/11 ,  G06T2207/10004 ,  G06T2207/30072 ,  Y10T436/115831 ,  Y10T436/117497 ,  C12Q2565/629 ,  C12Q2561/113

Abstract: The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system and to methods for monitoring real-time PCR in such systems.