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公开(公告)号:US20160003777A1
公开(公告)日:2016-01-07
申请号:US14771463
申请日:2014-02-14
IPC分类号: G01N29/06
CPC分类号: G01N29/0654 , G01N29/2418 , G01N29/44
摘要: The invention relates to a recording device comprising an excitation module (2, 10; 9, 10) which stimulates the sample (3) for emitting pressure waves, an acoustic module (9, 10) for detecting the generated pressure waves, and a control module (10) which determines an acoustic image based on the data from the acoustic module (9, 10). Said recording device also comprises a reproduction module (5) for optically reproducing the sample (3) and the control module (10) determines a sample limit and/or a segment limit within the sample (3) based on the optical reproduction of the sample (3) and when the acoustic image is detected, the determined sample limit and/or segment limit are taken into consideration.
摘要翻译: 本发明涉及一种记录装置,包括:激发模块(2,10; 9,10),其激发用于发射压力波的样品(3);用于检测产生的压力波的声学模块(9,10);以及控制 模块(10),其基于来自声学模块(9,10)的数据确定声学图像。 所述记录装置还包括用于光学地再现样品(3)的再现模块(5),并且控制模块(10)基于样品的光学再现来确定样品(3)内的样品极限和/或片段极限 (3),并且当检测到声学图像时,考虑所确定的样本极限和/或分段极限。
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公开(公告)号:US20130314758A1
公开(公告)日:2013-11-28
申请号:US13902763
申请日:2013-05-24
IPC分类号: G02B21/00
CPC分类号: G02B21/0044 , G02B21/0052 , G02B21/008
摘要: A light scanning microscope with an illumination module generates several illumination beams and moves them, in each case as a spot, in a predefined region of a sample to excite sample radiation. A detector module for confocal detection of the sample radiation excited by each spot includes a first detector, an imaging lens system, having an optical axis, for imaging the predefined region along an imaging beam path running from the sample as far as the first detector, and a rotatable diaphragm with several pinholes located in a pinhole plane. The diaphragm, upon rotation, may be located at least partially in the imaging beam path for confocal detection. A second detector may be arranged outside of the imaging beam path. A first beam splitter may be arranged in the imaging beam path between the sample and the diaphragm. The beam splitter deflects sample radiation onto the second detector.
摘要翻译: 具有照明模块的光扫描显微镜产生几个照明光束,并将它们在每种情况下作为点移动到样品的预定区域以激发样品辐射。 用于由每个点激发的样品辐射的共焦检测的检测器模块包括具有光轴的第一检测器,成像透镜系统,用于沿着从样品运行的成像光束路径成像到第一检测器, 以及具有位于针孔平面中的几个针孔的可旋转隔膜。 膜片在旋转时可以至少部分地位于用于共焦检测的成像光束路径中。 第二检测器可以布置在成像光束路径的外部。 第一分束器可以布置在样品和隔膜之间的成像光束路径中。 分束器将样品辐射器偏转到第二检测器上。
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公开(公告)号:US09733160B2
公开(公告)日:2017-08-15
申请号:US14132988
申请日:2013-12-18
发明人: Wibke Hellmich , Benno Radt , Helmut Lippert , Olaf Selchow , Uwe Wolf , Juergen Haese
摘要: The invention is directed to method for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement. According to the invention, the method mentioned above has the following method steps: a sample is introduced into a transparent medium, preferably agarose gel, which is initially liquid; the medium is changed from the liquid state to the solid state, wherein the sample is fixated within the medium, but the transparency of the medium is retained; the solidified medium is positioned in the microscope arrangement in such a way that the sample enclosed therein is situated in the detection area of the objective. Further, a device is proposed for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement.
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