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公开(公告)号：US09765292B2
公开(公告)日：2017-09-19
申请号：US14406043
申请日：2013-06-04
发明人： Elia Piccinini , David Wendt , Adam Papadimitropoulos , Laura Spinelli , Stefania Adele Riboldi , Francesco Giovanni Greco , Peter Iwatschenko
CPC分类号： C12M23/40 , A61L27/3847 , A61L27/3895 , C12M23/00 , C12M25/14 , C12M29/00 , C12N5/0062 , C12N5/0663 , C12N2533/14
摘要： A bioreactor for preferably three-dimensional cell culturing comprises a scaffold chamber, a first tube, a second tube and a first valve with a scaffold adapter, a tube adapter and a medium adapter. The first valve has a housing with a longitudinal female portion ending in an opening and a longitudinal actuator being arranged through the opening of the female portion of the housing such that the actuator is arranged partially inside the housing and partially outside the housing, wherein the actuator of the first valve is axially moveable relative to the housing of the first valve between a first position in which the first valve is in the operation position and a second position in which the first valve is in the medium change position. By providing the actuators in the first valve which is applied by axial movements, operation of the bioreactor can be comparably simple and safe.
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公开(公告)号：US20150197719A1
公开(公告)日：2015-07-16
申请号：US14406043
申请日：2013-06-04
发明人： Elia Piccinini , David Wendt , Adam Papadimitropoulos , Laura Spinelli , Stefania Adele Riboldi , Francesco Giovanni Greco , Peter Iwatschenko
CPC分类号： C12M23/40 , A61L27/3847 , A61L27/3895 , C12M23/00 , C12M25/14 , C12M29/00 , C12N5/0062 , C12N5/0663 , C12N2533/14
摘要： A bioreactor for preferably three-dimensional cell culturing comprises a scaffold chamber, a first tube, a second tube and a first valve with a scaffold adapter, a tube adapter and a medium adapter. The first valve has a housing with a longitudinal female portion ending in an opening and a longitudinal actuator being arranged through the opening of the female portion of the housing such that the actuator is arranged partially inside the housing and partially outside the housing, wherein the actuator of the first valve is axially moveable relative to the housing of the first valve between a first position in which the first valve is in the operation position and a second position in which the first valve is in the medium change position. By providing the actuators in the first valve which is applied by axial movements, operation of the bioreactor can be comparably simple and safe. In particular, such arrangement allows for a comparably easy identification of the position of the first valve, i.e., e.g., if the valve is in the medium change position or in the operation position. Furthermore, it also allows for exactly adjusting the position of the actuator such that also positions in between the medium change position and the operation position can be conveniently applied if necessary.
摘要翻译： 用于优选三维细胞培养的生物反应器包括支架室，第一管，第二管和具有支架适配器的第一阀，管适配器和介质适配器。 第一阀具有壳体，其具有终止于开口中的纵向阴部分并且纵向致动器布置成穿过壳体的阴部分的开口，使得致动器部分地布置在壳体内部并且部分地位于壳体外部，其中致动器 的第一阀在第一阀位于操作位置的第一位置和第一阀处于介质更换位置的第二位置之间相对于第一阀的壳体可轴向移动。 通过在通过轴向运动施加的第一阀中提供致动器，生物反应器的操作可以相当简单和安全。 特别地，这种布置允许比较容易地识别第一阀的位置，即例如，如果阀处于介质更换位置或操作位置。 此外，还可以精确地调节致动器的位置，使得如果需要也可以方便地应用介质更换位置和操作位置之间的位置。
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公开(公告)号：US10473646B2
公开(公告)日：2019-11-12
申请号：US15314245
申请日：2015-05-26
发明人： Christian Hirt , Adam Papadimitropoulos , Giandomenica Iezzi , Volker Lorber , Manuele Giuseppe Muraro
摘要： The invention relates to a method of in vitro culturing or expanding human or animal tissue (2, 2′). The method comprises: obtaining a sample of human or animal tissue; downsizing tissue (21, 21′) of the sample; generating an assembly by placing the downsized tissue on a scaffold or hydrogel (22, 22′); arranging the assembly inside a culture chamber of a 3D perfusion system (23, 23′); and perfusing the assembly in the 3D perfusion system for a predefined time (24, 24′). The method according to the invention allows for preparing and directly culturing in vitro fresh tissue specimens for a high efficient in vitro culturing using a perfused bioreactor system. As single cells of tissue are in most cases undergoing to an in vitro cell-death due to missing micro environmental signals, the use of downsized tissue, comprising cell clumps and tissue fragments of hundreds of cells, may help to prevent cell death in vitro as well as to maintain the initial heterogeneous tissue microenvironment consisting of specific cell types such as epithelial, stromal and immune cells together. Thus, the method according to the invention is capable of efficiently culturing the whole tissue over time, thus allowing for survival of original cell types.
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