公开(公告)号：US10233440B2

公开(公告)日：2019-03-19

申请号：US14431243

申请日：2013-09-26

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Reuel Van Atta ,  Kenneth E. Ho

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6886 ,  G06F19/20 ,  G01N1/06

Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.

公开(公告)号：US11299726B2

公开(公告)日：2022-04-12

申请号：US16255659

申请日：2019-01-23

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Reuel Van Atta ,  Kenneth E. Ho

IPC: C12Q1/68 ,  C12N15/10 ,  G16B25/00 ,  G16B25/10 ,  C12Q1/6806 ,  C12Q1/6886 ,  G01N1/06

Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.

公开(公告)号：US20190225959A1

公开(公告)日：2019-07-25

申请号：US16255659

申请日：2019-01-23

Applicant: Cepheid

Inventor： Edwin Wei-Lung Lai ,  Reuel Van Atta ,  Kenneth E. Ho

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6886 ,  G16B25/00

Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.

公开(公告)号：US10465182B2

公开(公告)日：2019-11-05

申请号：US15208525

申请日：2016-07-12

Applicant: Cepheid

Inventor： Kenneth E. Ho

IPC: C12N15/10 ,  C12Q1/6876 ,  C12N1/06 ,  C12Q1/6806 ,  C12Q1/686

Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a cell or tissue sample. In certain embodiments the sample comprises a formalin fixed paraffin embedded sample (e.g., a FFPET sample), or a fine needle aspirate and/or a cell/tissue smear. In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.

公开(公告)号：US20150252354A1

公开(公告)日：2015-09-10

申请号：US14431243

申请日：2013-09-26

Applicant: CEPHEID

Inventor： Edwin Wei-Lung Lai ,  Reuel Vanatta ,  Kenneth E. Ho

IPC: C12N15/10 ,  G06F19/20 ,  C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N1/06 ,  G06F19/20

Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.

Abstract translation: 提供方法和试剂用于从固定石蜡包埋样品（例如，FFPET样品）中快速提取核酸。 在一些实施方案中，所述方法包括将所述组织样品的一个或多个切片孵育在包含足以将所述溶液的pH保持在约pH 4至约pH 9范围内的pH的溶解溶液中; 离液剂; 螯合剂; 和洗涤剂; 其中孵育温度为约50℃至约100℃; 并从所述裂解液中回收核酸。