公开(公告)号：US12130253B2

公开(公告)日：2024-10-29

申请号：US17271164

申请日：2020-08-24

Applicant: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Zhong Cao ,  Junyi He ,  Qin Zhu ,  Chu Liu ,  Saifei Feng ,  Huiying Hu ,  Donghong Yu

IPC: G01N27/327 ,  C01B32/174 ,  G01N27/48

CPC classification number: G01N27/3278 ,  C01B32/174 ,  G01N27/48 ,  C01B2202/06 ,  C01B2202/22

Abstract: A method for a selective detection of L-tryptophan (L-Trp) using formaldehyde as a medium is disclosed. The method includes preparation of a copper sulfide nanosheets-chitosan/acidified functionalized multi-wall carbon nanotubes (CuS NS—CS/F-MWCNTs) composite material, preparation of a composite film-modified electrode CuS NS—CS/F-MWCNTs/GCE, and a detection of the L-Trp. Since oxidation peaks of L-Trp and L-tyrosine (L-Tyr) overlap and are difficult to separate, the present invention provides a method for a highly selective detection of L-Trp through the Pictet-Spengler reaction of formaldehyde (HCHO) with L-Trp, in which the oxidation peak potential of L-Trp is shifted to 0.82 V and the oxidation peak potential of L-Tyr is 0.63 V, thereby effectively avoiding the interference of L-Tyr. The CuS NS—CS/F-MWCNTs/GCE is applied to detect L-Trp in the formaldehyde medium without any interference from L-Tyr or other amino acids with 50-fold concentrations.