公开(公告)号：US12130253B2

公开(公告)日：2024-10-29

申请号：US17271164

申请日：2020-08-24

申请人： CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Zhong Cao ,  Junyi He ,  Qin Zhu ,  Chu Liu ,  Saifei Feng ,  Huiying Hu ,  Donghong Yu

IPC分类号： G01N27/327 ,  C01B32/174 ,  G01N27/48

CPC分类号： G01N27/3278 ,  C01B32/174 ,  G01N27/48 ,  C01B2202/06 ,  C01B2202/22

摘要： A method for a selective detection of L-tryptophan (L-Trp) using formaldehyde as a medium is disclosed. The method includes preparation of a copper sulfide nanosheets-chitosan/acidified functionalized multi-wall carbon nanotubes (CuS NS—CS/F-MWCNTs) composite material, preparation of a composite film-modified electrode CuS NS—CS/F-MWCNTs/GCE, and a detection of the L-Trp. Since oxidation peaks of L-Trp and L-tyrosine (L-Tyr) overlap and are difficult to separate, the present invention provides a method for a highly selective detection of L-Trp through the Pictet-Spengler reaction of formaldehyde (HCHO) with L-Trp, in which the oxidation peak potential of L-Trp is shifted to 0.82 V and the oxidation peak potential of L-Tyr is 0.63 V, thereby effectively avoiding the interference of L-Tyr. The CuS NS—CS/F-MWCNTs/GCE is applied to detect L-Trp in the formaldehyde medium without any interference from L-Tyr or other amino acids with 50-fold concentrations.

公开(公告)号：US11761922B2

公开(公告)日：2023-09-19

申请号：US16969588

申请日：2019-12-31

申请人： CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Zhong Cao ,  Ningtao Zhou ,  Li Zhou ,  Qin Zhu ,  Jinglin He ,  Zhongliang Xiao ,  Yumin He ,  Zemeng Feng ,  Yulong Yin

IPC分类号： G01N27/327 ,  C07K1/107 ,  G01N27/48

CPC分类号： G01N27/3277 ,  C07K1/1077 ,  G01N27/48

摘要： A method and a sensor for detecting L-arginine are provided. The method includes synthesizing ferrocene-functionalized hexadecapeptide dithiocyclopentane (FC-P16 Peptide), preparing a polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE), detecting L-Arg and other steps. The results show that the polypeptide composite membrane-modified electrode (FC-P16 Peptide/AuE) exhibits excellent electrochemical response properties to L-Arg. In 10 mmol/L phosphate-buffered saline (PBS, pH=7.4), the DPV response peak current of the polypeptide composite membrane-modified electrode has an excellent linear relationship with the L-Arg concentration of 1.0×10−13 mol/L to 1.0×10−7 mol/L, with a detection limit of 1.0×10−13 mol/L. With prominent reproducibility, repeatability and selectivity, the modified electrode has potential application in life science and nutritional health.

公开(公告)号：US11079353B2

公开(公告)日：2021-08-03

申请号：US16955797

申请日：2019-08-24

申请人： CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Zhong Cao ,  Yuyu Peng ,  Chen Liu ,  Ling Tang ,  Qin Zhu ,  Min Jin ,  Jinglin He ,  Zhongliang Xiao

IPC分类号： G01N27/414

摘要： A method and a sensor for detecting L-cysteine based on a 3,3′-dithiobis (1-propanesulfonate)-mercury composite membrane are provided. The method includes: implanting a p-well and an N-type substrate on field effect transistor substrate, constructing a source electrode and a drain electrode at the p-well, constructing a silicon dioxide layer on the substrate, plating an aluminum-copper alloy layer, a chromium-palladium alloy layer, and a gold film layer on a polysilicon gate substrate, constructing a silicon nitride layer on the substrate and the silicon dioxide layer; extending a gate part; preparing an ethanol solution of disodium 3,3′-dithiobis (1-propanesulfonate), cleaning and soaking gate gold electrode in the solution, followed by soaking in an ethanol solution of mercuric nitrate, and then washing the gate gold electrode; connecting electrode interfaces, inserting the electrode into PBS, and connecting power interfaces to a stabilized voltage supply, connecting a signal output interface to a multimeter.