公开(公告)号：US20220235320A1

公开(公告)日：2022-07-28

申请号：US17617480

申请日：2020-06-02

Applicant: CORNING INCORPORATED

Inventor： Martial Hervy ,  Liepin Huang ,  Jinlin Peng ,  Ryann Loren Russell ,  Jeffery Joseph Scibek ,  Yue Zhou

IPC: C12N5/00 ,  C08B37/00 ,  C08J3/075 ,  C08L5/06

Abstract: A cell culture article is provided. The cell culture article includes a substrate of a polygalacturonic acid compound crosslinked with a divalent cation, the polygalacturonic acid compound being selected from at least one of: pectic acid; partially esterified pectic acid, partially amidated pectic acid and salts thereof. The substrate is digestible by digestion reagents into components including of galacturonic acid monomers and the divalent cation. Methods of harvesting cells from the cell culture article are also provided. The methods include performing a series of wash and/or centrifugation cycles to reduce components in the harvest solution.