公开(公告)号：US20220235320A1

公开(公告)日：2022-07-28

申请号：US17617480

申请日：2020-06-02

Applicant: CORNING INCORPORATED

Inventor： Martial Hervy ,  Liepin Huang ,  Jinlin Peng ,  Ryann Loren Russell ,  Jeffery Joseph Scibek ,  Yue Zhou

IPC: C12N5/00 ,  C08B37/00 ,  C08J3/075 ,  C08L5/06

Abstract: A cell culture article is provided. The cell culture article includes a substrate of a polygalacturonic acid compound crosslinked with a divalent cation, the polygalacturonic acid compound being selected from at least one of: pectic acid; partially esterified pectic acid, partially amidated pectic acid and salts thereof. The substrate is digestible by digestion reagents into components including of galacturonic acid monomers and the divalent cation. Methods of harvesting cells from the cell culture article are also provided. The methods include performing a series of wash and/or centrifugation cycles to reduce components in the harvest solution.

公开(公告)号：US10900021B2

公开(公告)日：2021-01-26

申请号：US15579776

申请日：2016-06-08

Applicant: Corning Incorporated

Inventor： Paula Jean Dolley-Sonneville ,  David Henry ,  Jeffery Joseph Scibek ,  Yue Zhou

IPC: C12N5/0775 ,  C12N5/00

Abstract: A method of making a cell culture article is provided. The method includes forming a microcarrier from a microcarrier composition comprising a polygalacturonic acid compound or an alginic acid compound, infiltrating the microcarrier with a drying formulation to form an infiltrated microcarrier, and drying the infiltrated microcarrier to form a dried microcarrier, wherein the drying formulation comprises at least one of a saccharide and a monovalent cation.

公开(公告)号：US20200277560A1

公开(公告)日：2020-09-03

申请号：US16764149

申请日：2018-11-29

Applicant: CORNING INCORPORATED

Inventor： Jeffery Joseph Scibek

IPC: C12M1/26 ,  C12M1/00 ,  C12M1/02 ,  C12M1/12 ,  C12N5/07

Abstract: A bioreactor is provided herein. The bioreactor includes a vessel having a wall at least partially defining an interior compartment for receiving fluid, at least one port, and at least one cap configured to removably engage with the at least one port, the at least one cap comprising a filter material. A cell culture method is also provided herein which includes adding cells and cell growth medium to a vessel of a bioreactor and adding microcarriers to the vessel to form substantially confluent cells on the microcarriers. The cell culture method further includes washing the confluent cells, harvesting the confluent cells to form a solution containing the cells, and removing the solution containing the cells from the vessel by flowing the solution through a filter material in a cap removably engaged with at least one port of the bioreactor.

公开(公告)号：US20210207098A1

公开(公告)日：2021-07-08

申请号：US17126348

申请日：2020-12-18

Applicant: CORNING INCORPORATED

Inventor： Paula Jean Dolley-Sonneville ,  David Henry ,  Jeffery Joseph Scibek ,  Yue Zhou

IPC: C12N5/0775 ,  C12N5/00

Abstract: A method of making a cell culture article is provided. The method includes forming a microcarrier from a microcarrier composition comprising a polygalacturonic acid compound or an alginic acid compound, infiltrating the microcarrier with a drying formulation to form an infiltrated microcarrier, and drying the infiltrated microcarrier to form a dried microcarrier, wherein the drying formulation comprises at least one of a saccharide and a monovalent cation.

公开(公告)号：US10920185B2

公开(公告)日：2021-02-16

申请号：US16094233

申请日：2017-05-31

Applicant: Corning Incorporated

Inventor： Jeffery Joseph Scibek ,  James Clark Walck, Jr.

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/06 ,  C12M1/24 ,  B01F13/08 ,  B01F7/00

Abstract: A vessel for culturing cells is provided that includes a vessel body having a top portion, a bottom portion comprising a bottom interior surface, and a cylindrical sidewall. The vessel additionally has an impeller assembly inside the vessel body having a top portion rotatably coupled to the top portion of the vessel body, the impeller assembly having a plurality of planar blades, a central axis, a flexible shaft extending down from the top portion of the impeller assembly, a magnet receptacle molded within the plurality of planar blades, a magnet within the magnet receptacle, and an impeller o-ring coupled to a bottom surface of the planar blades. The vessel also includes a plurality of positioning nubs coupled to the bottom interior surface of the vessel body, spaced from an inside edge of the impeller o-ring.

公开(公告)号：US20190112567A1

公开(公告)日：2019-04-18

申请号：US16094233

申请日：2017-05-31

Applicant: Corning Incorporated

Inventor： Jeffery Joseph Scibek ,  James Clark Walck, JR.

IPC: C12M1/06 ,  C12M1/24 ,  C12M1/00 ,  B01F13/08

Abstract: A vessel for culturing cells is provided that includes a vessel body having a top portion, a bottom portion comprising a bottom interior surface, and a cylindrical sidewall. The vessel additionally has an impeller assembly inside the vessel body having a top portion rotatably coupled to the top portion of the vessel body, the impeller assembly having a plurality of planar blades, a central axis, a flexible shaft extending down from the top portion of the impeller assembly, a magnet receptacle molded within the plurality of planar blades, a magnet within the magnet receptacle, and an impeller o-ring coupled to a bottom surface of the planar blades. The vessel also includes a plurality of positioning nubs coupled to the bottom interior surface of the vessel body, spaced from an inside edge of the impeller o-ring.