公开(公告)号：US20220242903A1

公开(公告)日：2022-08-04

申请号：US17616577

申请日：2020-06-11

Applicant: CYTIVA SWEDEN AB

Inventor： Niklas JUNGNELIUS ,  Andreas CASTAN

IPC: C07K1/36 ,  C12M1/00 ,  C12M1/34 ,  C07K1/22 ,  B01D15/20 ,  B01D15/42 ,  B01D15/18

Abstract: The present disclosure is directed to method (100) for separating a biomolecule from a fluid (2), wherein the biomolecule is produced by biological cells in a perfusion bioreactor (3), the method comprising: forwarding (110) a product fluid comprising the biomolecule and biological cells from the perfusion bioreactor (3) during a bioreactor product output time period; loading (210) a first separation device (4) with the product fluid obtained in step (110) during a first separation loading time period, which constitutes a first part of a first separation cycle time period; pausing (130) the forwarding of the product fluid from the perfusion bioreactor during a bioreactor non-product output time period; and pausing (220) the loading of the first separation device (4) during a second part of the first separation cycle time period. Also disclosed are a computer-implemented method performed by a controller configured to control a separation of a biomolecule from a fluid (2), and further a controller, a computer program, and a computer program product.