-
公开(公告)号：US11927503B2
公开(公告)日：2024-03-12
申请号：US17276981
申请日：2019-09-09
Applicant: Cytiva Sweden AB
Inventor： Sameera Simha Hindupur , Manoj Kumar Ramakrishna , Andreas Castan , Swapnil Puranik , Klaus Gebauer
CPC classification number: G01M3/2884 , B29C65/8246 , B29C66/522 , C12M23/40 , C12M37/04 , C12M41/40
Abstract: The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.
-
公开(公告)号：US12182482B2
公开(公告)日：2024-12-31
申请号：US16620940
申请日：2018-06-18
Applicant: Cytiva Sweden AB
Inventor： Krishna Kumar Swaminathan , Sridhar Dasaratha , Sittal George , Vinay Bhaskar Jammu , Alok Singh Chauhan , Nagaraju Konduru , Neelima Boddapati , Ankita Bhatia , Mithilesh Mohanty , Harsha Aeron , Andreas Castan
IPC: G06F30/20
Abstract: The present invention relates to a method for predicting outcome of a process used for manufacturing a sample in a bioreactor, the process belonging to a category. The method comprises selecting (51) a process model based on the category; accessing (53) historic data related to past process runs for manufacturing the sample; accessing (53) current data obtained (54) from a current process run of the process. The obtained current data, which is based on the selected process model, comprises: process strategy data, bioreactor instrument data, data from online sensors and/or data from offline sensors. The method further comprises predicting (62) an outcome of at least one selected parameter of the current process run for manufacturing the sample based on the accessed historic data and current data. The present invention also relates to a method for modelling a process and a control system (10) for controlling a process.
-
公开(公告)号：US20220127557A1
公开(公告)日：2022-04-28
申请号：US17435436
申请日：2020-03-25
Applicant: Cytiva Sweden AB
Inventor： Andreas Castan , Ricardo A. Medronho , Leda Castilho , Iona Walter Bettinardi
Abstract: A bioreactor system for culturing cells, comprising: —at least one bioreactor (3); and —at least one hydrocyclone (5a; 5b; 5c; 5d, 5d′; 5e) comprising: —a cell culture inlet (7) which is connected to a cell culture outlet (9) of the bioreactor (3) via a pump (11), whereby a cell culture from the bioreactor can be transferred to the hydrocyclone (5a: 5b; 5c; 5d, 5d′; 5e) when a cell culture is provided in the bioreactor, —a hydrocyclone separation part (13) into which cell culture can be introduced from the cell culture inlet (7), —a top outlet (15) through which a part of the cell culture with decreased cell concentration can be transferred after separation in the hydrocyclone separation pan (13); and —a bottom outlet (17) through which a pan of the cell culture with increased cell concentration can be transferred after separation in the hydrocyclone separation part (13), wherein said bottom outlet (17) of the hydrocyclone (5a; 5b; 5c; 5d, 5d′; 5e) is provided in a position such that when the bioreactor system is used for culturing cells the bottom outlet (17) is in connection with a headspace (21) of the bioreactor (3) and wherein said bottom outlet (17) is provided such that the bottom outlet (17) has a free space around it such that a cell culture transferred out form the bottom outlet (17) is allowed to freely discharge around the bottom outlet.
-
公开(公告)号：US11549091B2
公开(公告)日：2023-01-10
申请号：US14910824
申请日：2013-08-27
Applicant: Cytiva Sweden AB
Inventor： Andreas Castan , Klaus Gebauer
IPC: C12M1/00 , C12M1/06 , C12M3/00 , C12M3/06 , C12N1/16 , C12N1/20 , C12N5/04 , C12N5/07 , C12N5/071
Abstract: The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.
-
公开(公告)号：US20220034748A1
公开(公告)日：2022-02-03
申请号：US17276981
申请日：2019-09-09
Applicant: Cytiva Sweden AB
Inventor： Sameera Simha Hindupur , Manoj Kumar Ramakrishna , Andreas Castan , Swapnil Puranik , Klaus Gebauer
Abstract: The present invention relates to a computer implemented method for determining a weld integrity test result performed by a system configured to aseptically transferring cells to a container (BR), the method comprising welding (710) a vial to a connector to obtain a fluid-tight seal between the vial (V) and a connector (C), the connector being provided with a pair of conduits each having one end protruding through the connector and into the vial, sealing (720) an opposite end of one of the conduits, generating (730) a fluid pressure different to an ambient fluid pressure at an opposite end of the other conduit, measuring (740) a fluid pressure within at least one of the conduits, determining (750) a fluid pressure change based on the ambient pressure and the measured fluid pressure, determining (760) the weld integrity test result as pass or as fail based on the fluid pressure change.
-
Patent Agency Ranking
公开(公告)号：US20250068794A1
公开(公告)日：2025-02-27
申请号：US18948020
申请日：2024-11-14
Applicant: Cytiva Sweden AB
Inventor： Krishna Kumar Swaminathan , Sridhar Dasaratha , Sittal George , Vinay Bhaskar Jammu , Alok Singh Chauhan , Nagaraju Konduru , Neelima Boddapati , Ankita Bhatia , Mithilesh Mohanty , Harsha Aeron , Andreas Castan
IPC: G06F30/20
Abstract: A method for predicting outcome of a process used for manufacturing a sample in a bioreactor, the process belonging to a category. The method comprises selecting a process model based on the category; accessing historic data related to past process runs for manufacturing the sample; accessing current data obtained from a current process run of the process. The obtained current data, which is based on the selected process model, comprises: process strategy data, bioreactor instrument data, data from online sensors and/or data from offline sensors. The method further comprises predicting an outcome of at least one selected parameter of the current process run for manufacturing the sample based on the accessed historic data and current data.
-
公开(公告)号：US20240043879A1
公开(公告)日：2024-02-08
申请号：US18467658
申请日：2023-09-14
Applicant: Cytiva Sweden AB
Inventor： Daniel Ivansson , Andreas Castan
CPC classification number: C12N15/907 , C12P21/00 , C07K16/00 , C12N2510/02
Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells.
The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.-
公开(公告)号：US20230093940A1
公开(公告)日：2023-03-30
申请号：US18062481
申请日：2022-12-06
Applicant: Cytiva Sweden AB
Inventor： Andreas Castan , Klaus Gebauer
IPC: C12M1/00 , C12N5/07 , C12M3/06 , C12M3/00 , C12M1/06 , C12N5/071 , C12N1/20 , C12N5/04 , C12N1/16
Abstract: The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.
-
公开(公告)号：US11525111B2
公开(公告)日：2022-12-13
申请号：US16586272
申请日：2019-09-27
Applicant: Cytiva Sweden AB
Inventor： Klaus Gebauer , Andreas Castan , Camilla Estmer-Nilsson , Thomas Falkman , Anders Wilen , Michael Miller , Ralph Stankowski
Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.
-
公开(公告)号：US11136598B2
公开(公告)日：2021-10-05
申请号：US15907470
申请日：2018-02-28
Applicant: Cytiva Sweden AB
Inventor： Daniel Leif Ivansson , Andreas Castan
Abstract: The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GO genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.
-
-
-
-
-
-
-
-
-
Search Results
10
records
(took 0.015 seconds)
