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1.发明授权RECOMBINANT PROTEINS OF FILAMENTOUS HAEMAGGLUTININ OF BORDETELLA, PARTICULARLY BORDETELLA PERTUSSIS, METHOD FOR PRODUCING SAME, AND USES THEREOF FOR PRODUCING FOREIGN PROTEINS OF VACCINATING ACTIVE PRINCIPLES 失效BORDETELLA，特别是BORDETELLA PERTUSSIS，其生产方法的FILAMENTOUS HAEMAGGLUTININ的重组蛋白及其用于生产外源性活性原子蛋白的用途公开(公告)号：US06841358B1
公开(公告)日：2005-01-11
申请号：US08765287
申请日：1995-04-19
申请人： Camille Locht , Genevieve Renauld , Andre Capron , Gilles Riveau , Franco Menozzi , Francoise Jacob-Dubuisson
发明人： Camille Locht , Genevieve Renauld , Andre Capron , Gilles Riveau , Franco Menozzi , Francoise Jacob-Dubuisson
CPC分类号： C07K14/235 , C07K2319/00
摘要： A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the reading frame to a sequence (2) located upstream from the first sequence. Sequence (2) codes for at least part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein, which contains the interaction site of Fha and heparin and the secretion domain. This Fha protein is under the control of a promoter recognized by the cell polymerases of B. pertussis and is inserted into a B. pertussis cell culture, is expressed in the culture and excreted into the cell culture medium. The invention uses both the abilities of Bordetella and particularly B. pertussis to secrete or surface expose the heterologous polypeptide fused to the Fha portion corresponding to sequence (2), which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from other Bordetella proteins.
摘要翻译： 一种重组DNA，其含有编码与阅读框内融合到位于第一序列上游的序列（2）的博德氏菌（Fha）的丝状血凝素异源的多肽的序列（1）。 序列（2）编码Fha前体的至少一部分，其部分至少包含截短的成熟Fha蛋白的N末端区域，其含有Fha和肝素的相互作用位点和分泌结构域。 该Fha蛋白质受由百日咳杆菌的细胞聚合酶识别的启动子的控制，并插入百日咳杆菌细胞培养物中，在培养物中表达并分泌到细胞培养基中。 本发明既使用博德特氏菌，特别是百日咳博德特氏菌分泌或表面暴露融合到对应于序列（2）的Fha部分的异源多肽（其似乎不产生细胞外蛋白酶），以及丝状血凝素可以容易地 与其他博德特氏蛋白分离。
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