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    RECOMBINANT PROTEINS OF FILAMENTOUS HAEMAGGLUTININ OF BORDETELLA, PARTICULARLY BORDETELLA PERTUSSIS, METHOD FOR PRODUCING SAME, AND USES THEREOF FOR PRODUCING FOREIGN PROTEINS OF VACCINATING ACTIVE PRINCIPLES
    1.
    发明授权
    RECOMBINANT PROTEINS OF FILAMENTOUS HAEMAGGLUTININ OF BORDETELLA, PARTICULARLY BORDETELLA PERTUSSIS, METHOD FOR PRODUCING SAME, AND USES THEREOF FOR PRODUCING FOREIGN PROTEINS OF VACCINATING ACTIVE PRINCIPLES 失效
    BORDETELLA，特别是BORDETELLA PERTUSSIS，其生产方法的FILAMENTOUS HAEMAGGLUTININ的重组蛋白及其用于生产外源性活性原子蛋白的用途

    公开(公告)号：US06841358B1

    公开(公告)日：2005-01-11

    申请号：US08765287

    申请日：1995-04-19

    申请人： Camille Locht ,  Genevieve Renauld ,  Andre Capron ,  Gilles Riveau ,  Franco Menozzi ,  Francoise Jacob-Dubuisson

    发明人： Camille Locht ,  Genevieve Renauld ,  Andre Capron ,  Gilles Riveau ,  Franco Menozzi ,  Francoise Jacob-Dubuisson

    IPC分类号： C07K14/235 ,  C12N15/31 ,  C12N15/09 ,  A01N63/00 ,  A61K39/00 ,  A61K39/02 ,  C12P21/06

    CPC分类号： C07K14/235 ,  C07K2319/00

    摘要： A recombinant DNA containing a sequence (1) coding for a polypeptide heterologous to a filamentous haemagglutinin of Bordetella (Fha) fused within the reading frame to a sequence (2) located upstream from the first sequence. Sequence (2) codes for at least part of the Fha precursor, which part comprises at least the N-terminal region of a truncated mature Fha protein, which contains the interaction site of Fha and heparin and the secretion domain. This Fha protein is under the control of a promoter recognized by the cell polymerases of B. pertussis and is inserted into a B. pertussis cell culture, is expressed in the culture and excreted into the cell culture medium. The invention uses both the abilities of Bordetella and particularly B. pertussis to secrete or surface expose the heterologous polypeptide fused to the Fha portion corresponding to sequence (2), which does not appear to produce extracellular proteases, and the ease with which filamentous haemagglutinins can be isolated from other Bordetella proteins.

    摘要翻译： 一种重组DNA，其含有编码与阅读框内融合到位于第一序列上游的序列（2）的博德氏菌（Fha）的丝状血凝素异源的多肽的序列（1）。 序列（2）编码Fha前体的至少一部分，其部分至少包含截短的成熟Fha蛋白的N末端区域，其含有Fha和肝素的相互作用位点和分泌结构域。 该Fha蛋白质受由百日咳杆菌的细胞聚合酶识别的启动子的控制，并插入百日咳杆菌细胞培养物中，在培养物中表达并分泌到细胞培养基中。 本发明既使用博德特氏菌，特别是百日咳博德特氏菌分泌或表面暴露融合到对应于序列（2）的Fha部分的异源多肽（其似乎不产生细胞外蛋白酶），以及丝状血凝素可以容易地 与其他博德特氏蛋白分离。

    Bordetella strain expressing the FHA hybrid, liposomes and vaccines
    3.
    发明授权
    Bordetella strain expressing the FHA hybrid, liposomes and vaccines 失效
    表达FHA杂交体的Bordetella菌株，脂质体和疫苗

    公开(公告)号：US06660261B1

    公开(公告)日：2003-12-09

    申请号：US09284252

    申请日：1999-09-16

    申请人： Nathalie Mielcarek ,  Camille Locht ,  Gilles Riveau ,  Odile Poulain-Godefroy ,  André Capron

    发明人： Nathalie Mielcarek ,  Camille Locht ,  Gilles Riveau ,  Odile Poulain-Godefroy ,  André Capron

    IPC分类号： A01N6300

    CPC分类号： C12R1/01 ,  A61K39/00 ,  C07K14/235 ,  Y02A50/423

    摘要： The invention concerns a Bordetella strain deficient in the production of toxin and expressing a hybrid protein comprising at least part of the filamentous hemagglutin (FHA) and at least part of a protein heterologous to FHA. The gene coding for the toxin has been eliminated, or at least partially deleted or mutated so as to produce an inactive toxin. This strain can be used as vaccine. The invention also concerns liposomes containing at least part of the FHA protein and at least one protein heterologous to the FHA protein, and the use of FHA for the stimulation of immune responses.

    摘要翻译： 本发明涉及一种缺乏毒素生产的博德氏菌菌株，并表达一种杂合蛋白质，其包含至少部分丝状血凝素（FHA）和至少部分与FHA异源的蛋白质。 编码毒素的基因已被消除，或至少部分缺失或突变，以产生无活性毒素。 该菌株可用作疫苗。 本发明还涉及含有至少部分FHA蛋白质和至少一种与FHA蛋白质异源的蛋白质的脂质体，以及使用FHA来刺激免疫应答。