公开(公告)号：US20120156779A1

公开(公告)日：2012-06-21

申请号：US13392538

申请日：2010-08-23

申请人： Cecilia Anneren ,  Andreas Axen ,  Asa Bjurling ,  Christine Sund Lundstrom ,  James Van Alstine ,  Gunnar Froman

发明人： Cecilia Anneren ,  Andreas Axen ,  Asa Bjurling ,  Christine Sund Lundstrom ,  James Van Alstine ,  Gunnar Froman

IPC分类号： C12N5/02

CPC分类号： C12N5/0068 ,  C12N5/0018 ,  C12N2533/70

摘要： The present invention relates to a method for cell expansion. In the method, preferably a cell culture product is used, such as a microcarrier, or other adherent cell culture surface, comprising degradable polysaccharide, preferably starch, modified with small molecular weight cell-binding ligands. This allows recovery (detachment) of adhered cells to be aided by degradation of the culture surface with enzymatic agents, such as amylase. The method for cell expansion comprises the following steps: a) adding cells, culture medium and cell culture surface comprising a degradable polysaccharide with guanidine group containing ligands to a bioreactor; b) expanding said cells by adherent cell culture; and c) aiding the detachment of said cells by exposing them to a polysaccharidase to degrade the culturing surface.

摘要翻译： 本发明涉及一种细胞扩增方法。 在该方法中，优选使用细胞培养产物，例如微载体或其它贴壁细胞培养物表面，其包含用小分子量细胞结合配体修饰的可降解多糖，优选淀粉。 这允许通过用酶剂如淀粉酶降解培养物表面来辅助附着细胞的恢复（分离）。 细胞扩增方法包括以下步骤：a）将包含含有胍基团的可降解多糖的细胞，培养基和细胞培养物表面加入到生物反应器中; b）通过贴壁细胞培养来扩增所述细胞; 和c）通过将所述细胞暴露于多糖酶来降低所述培养表面来帮助所述细胞脱离。

公开(公告)号：US20120214230A1

公开(公告)日：2012-08-23

申请号：US13503388

申请日：2010-10-21

申请人： Cecilia Anneren ,  Gunnar Glad ,  Christine Sund Lundstrom

发明人： Cecilia Anneren ,  Gunnar Glad ,  Christine Sund Lundstrom

IPC分类号： C12N5/071 ,  C09D105/02 ,  C12M3/00

CPC分类号： C12M39/00 ,  C12M23/20 ,  C12M25/16

摘要： The present invention relates to a fast a simple coating procedure for coating of cell culture and/or handling surfaces to prevent cell adhesion and growth. The invention relates to a cell culture/handling product coated with phenyl dextran, as well as methods of producing and using it.

摘要翻译： 本发明涉及一种用于涂覆细胞培养和/或处理表面以防止细胞粘附和生长的简单涂层方法。 本发明涉及涂有苯基葡聚糖的细胞培养/处理产品，以及生产和使用它的方法。

公开(公告)号：US20110070648A1

公开(公告)日：2011-03-24

申请号：US12991930

申请日：2009-05-11

申请人： Cecilia Anneren ,  Linnea Pauler

发明人： Cecilia Anneren ,  Linnea Pauler

IPC分类号： C12N5/071 ,  C12N5/0775 ,  C12N5/078

CPC分类号： C12M25/16 ,  C12M23/14 ,  C12M23/20 ,  C12N5/0663 ,  C12N2531/00

摘要： The present invention relates to a method for cell expansion. More closely, it relates to a method for expansion of cells, such as mesenchymal stem cells, on microcarriers in a plastic bag bioreactor. The invention enables expansion to therapeutic amounts of stem cells. The method comprises the following steps: a) addition of cells in cell culture medium and microcarriers to a plastic bag container; b) allowing the cells to adhere to the microcarriers while the container is kept substantially still; c) addition of further cell culture medium once the cells have adhered; d) culturing the cells under gentle and constant agitation; e) increase the surface area for continued culturing; and f) final harvesting of cells by an active detachment and separation step.

摘要翻译： 本发明涉及一种细胞扩增方法。 更详细地说，它涉及在塑料袋生物反应器中的微载体上扩增细胞如间充质干细胞的方法。 本发明能够扩展至治疗量的干细胞。 该方法包括以下步骤：a）将细胞培养基和微载体中的细胞加入到塑料袋容器中; b）允许细胞在容器保持基本静止时粘附到微载体; c）一旦细胞粘附，加入另外的细胞培养基; d）在温和和持续搅拌下培养细胞; e）增加继续培养的表面积; 和f）通过主动分离和分离步骤最终收获细胞。