公开(公告)号：US20130326644A1

公开(公告)日：2013-12-05

申请号：US13916716

申请日：2013-06-13

Applicant: CELLECTIS, S.A.

Inventor： Frederic PAQUES

IPC: C12N9/16

CPC classification number: C12N9/16 ,  C12N9/22

Abstract: A LAGLIDADG homing endonuclease variant, having mutations in two separate subdomains, each binding a distinct part of a modified DNA target half-site, said LAGLIDADG homing endonuclease variant being able to cleave a chimeric DNA target sequence comprising the nucleotides bound by each subdomain. Use of said herodimeric meganuclease and derived products for genetic engineering, genome therapy and antiviral therapy.

Abstract translation: LAGLIDADG归巢内切核酸酶变体，在两个单独的子结构域中具有突变，每个结合修饰的DNA靶半位点的不同部分，所述LAGLIDADG归巢内切核酸酶变体能够切割包含由每个亚结构域结合的核苷酸的嵌合DNA靶序列。 使用所述的英雄二聚大范围核酸酶和衍生产品进行遗传工程，基因组治疗和抗病毒治疗。

公开(公告)号：US20130203840A1

公开(公告)日：2013-08-08

申请号：US13649093

申请日：2012-10-10

Applicant: Cellectis S.A.

Inventor： Sylvain ARNOULD ,  Patrick Chames ,  Philippe DUCHATEAU ,  Jean-Charles EPINAT ,  Emmanuel LACROIX ,  Frederic PAQUES

IPC: A61K48/00 ,  A61K38/17

CPC classification number: C12N15/907 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0337 ,  A61K38/00 ,  A61K38/1709 ,  A61K38/465 ,  A61K48/00 ,  A61K48/0058 ,  C07K14/435 ,  C07K2319/81 ,  C12N7/00 ,  C12N9/22 ,  C12N15/1058 ,  C12N15/8509 ,  C12N15/86 ,  C12N15/90 ,  C12N2730/10143 ,  C12N2799/022 ,  C12N2800/80 ,  C12N2830/002 ,  C12N2830/55 ,  C12N2840/20 ,  C12N2840/44 ,  C12Y301/00 ,  C12Y301/21004

Abstract: A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y in a single polypeptide.

Abstract translation: 单链归巢内切核酸酶，其包含具有登录号pdb 1g9y的氨基酸序列的I-CreI的第一变体和在单个多肽中具有登录号pdb 1g9y的氨基酸序列的I-CreI变体的第二变体。