公开(公告)号：US20050118591A1

公开(公告)日：2005-06-02

申请号：US10496410

申请日：2001-11-22

申请人： Cengiz Tamak ,  Alf-Andreas Krehan ,  Pia Steffens ,  Stefanie Waschuetza ,  Veit Zieglschmid

发明人： Cengiz Tamak ,  Alf-Andreas Krehan ,  Pia Steffens ,  Stefanie Waschuetza ,  Veit Zieglschmid

IPC分类号： C07K16/30 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C07K16/30 ,  G01N33/54326 ,  G01N33/56966 ,  G01N33/57492

摘要： The invention relates to a diagnosis kit, a DNA chip, and to methods for diagnosing or supervising the treatment of testicular cancer, which are of greatest importance, in particular, within the scope of cancer prevention and post-treatment. The invention is essentially characterized in that the mRNA of testicular tumor markers is detected in a blood sample, whereby the tumor markers depict a tumor-associated gene expression. To this end, particularly β-hCG, AFP, PLAP or GCAP come into question. The detection of the mRNA is carried out by reverse transcription in cDNA and by subsequently amplifying selected segments of the cDNA by means of polymerase chain reaction.

摘要翻译： 本发明涉及诊断试剂盒，DNA芯片，以及用于诊断或监督睾丸癌治疗的方法，其特别是在癌症预防和后处理的范围内是最重要的。 本发明的基本特征在于，在血液样品中检测到睾丸肿瘤标志物的mRNA，由此肿瘤标志物描绘肿瘤相关基因表达。 为此，特别是beta-hCG，AFP，PLAP或GCAP受到质疑。 mRNA的检测通过cDNA中的逆转录和随后通过聚合酶链反应扩增cDNA的选定区段来进行。

公开(公告)号：US20080206866A1

公开(公告)日：2008-08-28

申请号：US11909142

申请日：2006-03-22

申请人： Veit Zieglschmid ,  Oliver Bocher ,  Winfried Albert

发明人： Veit Zieglschmid ,  Oliver Bocher ,  Winfried Albert

IPC分类号： C12N5/06

CPC分类号： C12Q1/6806 ,  C12Q2600/158 ,  C12Q2527/127

摘要： The present invention relates to a reagent for prevention of time-dependent, induced expression in biological cells in a sample. It likewise relates to a method for this purpose and also to cell preparations treated in this manner, the reagent for prevention of time-dependence induced expression in biological cells in a sample ex vivo containing a formaldehyde donor.

摘要翻译： 本发明涉及用于预防样品中生物细胞中时间依赖性诱导表达的试剂。 它同样涉及用于此目的的方法以及以这种方式处理的细胞制剂，用于预防离体含有甲醛供体的样品中生物细胞中时间依赖性诱导表达的试剂。