公开(公告)号：US10688412B2

公开(公告)日：2020-06-23

申请号：US15659093

申请日：2017-07-25

Applicant: Cephalon, Inc.

Inventor： Lu Wang ,  Tianyi Zhou ,  Zhaoqing Zhang ,  Mi Jin

IPC: B01D15/38 ,  C07K1/22 ,  C07K14/555 ,  C07K16/18 ,  C07K16/28

Abstract: A wash buffer comprising greater than 0 mM and less than about 500 mM arginine, greater than 0 mM and less than about 250 mM guanidine, greater than 0 mM and less than about 250 mM sodium chloride, and greater than 0 mM and less than about 50 mM of an anionic surfactant, or greater than 0% and less than about 0.25% w/v of a non-ionic surfactant. When used during affinity chromatography purification of a protein of interest, such as an antibody, the wash buffer significantly reduces the level of host cell proteins from the preparation. Following affinity chromatography with the wash buffer, the protein of interest may be further purified using membrane chromatography.

公开(公告)号：US11426706B2

公开(公告)日：2022-08-30

申请号：US16014778

申请日：2018-06-21

Applicant: Cephalon, Inc.

Inventor： Lu Wang ,  Albert Kao ,  Zhaoqing Zhang ,  Mi Jin ,  Tianyi Zhou

IPC: B01D15/36 ,  C07K1/18 ,  B01J20/286 ,  B01J20/28 ,  C07K1/22 ,  C07K16/18 ,  C12Q1/686 ,  C07K16/00 ,  C07K16/28

Abstract: A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations.