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    Diagnostics based on tetrazolium compounds
    4.
    发明授权
    Diagnostics based on tetrazolium compounds 有权
    基于四唑化合物的诊断

    公开(公告)号：US06656697B1

    公开(公告)日：2003-12-02

    申请号：US09513071

    申请日：2000-02-25

    申请人： Tianmei Ouyang ,  Yeung Siu Yu

    发明人： Tianmei Ouyang ,  Yeung Siu Yu

    IPC分类号： G01N33553

    CPC分类号： C12Q1/32 ,  C12Q1/26 ,  C12Q1/54 ,  G01N33/525 ,  G01N33/64 ,  Y10S435/962 ,  Y10S435/97 ,  Y10T436/173076

    摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.

    摘要翻译： 试剂适合于测量含血红蛋白的生物液体如全血中的分析物的浓度。 该试剂包含对分析物具有特异性的黄素依赖性酶，如果并且仅当黄素与酶结合的话，黄素辅因子，四唑染料前体，电子转移剂和亚硝酸盐。 试剂导致染料形成，这是测量分析物浓度。 亚硝酸盐抑制由血红蛋白非酶促引起的干扰性染料形成。 优选地，试剂用于测量全血葡萄糖的干燥条中。

    Diagnostics based on tetrazolium compounds
    5.
    发明授权
    Diagnostics based on tetrazolium compounds 有权
    基于四唑化合物的诊断

    公开(公告)号：US06200773B1

    公开(公告)日：2001-03-13

    申请号：US09282083

    申请日：1999-03-30

    申请人： Tianmei Ouyang ,  Yeung Siu Yu

    发明人： Tianmei Ouyang ,  Yeung Siu Yu

    IPC分类号： C12Q132

    CPC分类号： G01N33/525 ,  C12Q1/32 ,  G01N33/64 ,  Y10S435/962 ,  Y10S435/97 ,  Y10T436/173076

    摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD, an NAD derivative, pyrrolo-quinoline quinone (PQQ), or a PQQ derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

    摘要翻译： 试剂适合于测量含血红蛋白的生物液体如全血中的分析物的浓度。 该试剂包含对分析物，NAD，NAD衍生物，吡咯并喹啉醌（PQQ）或PQQ衍生物，四唑染料前体，心肌黄酶或其类似物和亚硝酸盐具有特异性的脱氢酶。 试剂导致染料形成，这是测量分析物浓度。 亚硝酸盐抑制由血红蛋白非酶促引起的干扰性染料形成。 优选地，试剂用于测量酮体例如β-羟基丁酸酯的干燥条。

    Diagnostics based on tetrazolium compounds
    6.
    发明授权
    Diagnostics based on tetrazolium compounds 有权

    公开(公告)号：US07011954B2

    公开(公告)日：2006-03-14

    申请号：US10663217

    申请日：2003-09-15

    申请人： Tianmei Ouyang ,  Yeung Siu Yu

    发明人： Tianmei Ouyang ,  Yeung Siu Yu

    IPC分类号： G01N33/533

    CPC分类号： C12Q1/32 ,  C12Q1/26 ,  C12Q1/54 ,  G01N33/525 ,  G01N33/64 ,  Y10S435/962 ,  Y10S435/97 ,  Y10T436/173076

    摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.

    Diagnostics based on tetrazolium compounds
    7.
    发明授权
    Diagnostics based on tetrazolium compounds 有权
    基于四唑化合物的诊断

    公开(公告)号：US5902731A

    公开(公告)日：1999-05-11

    申请号：US161876

    申请日：1998-09-28

    申请人： Tianmei Ouyang ,  Yeung Siu Yu

    发明人： Tianmei Ouyang ,  Yeung Siu Yu

    IPC分类号： G01N33/50 ,  C12Q1/32 ,  C12Q1/54 ,  G01N33/52 ,  G01N33/64 ,  G01N33/66 ,  G01N33/68 ,  G01N33/72 ,  G01N33/49

    CPC分类号： G01N33/525 ,  C12Q1/32 ,  G01N33/64 ,  Y10S435/962 ,  Y10S435/97 ,  Y10T436/173076

    摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD or an NAD derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

    摘要翻译： 试剂适合于测量含血红蛋白的生物液体如全血中的分析物的浓度。 该试剂包含对分析物，NAD或NAD衍生物，四唑染料前体，心肌黄酶或其类似物和亚硝酸盐具有特异性的脱氢酶。 试剂导致染料形成，这是测量分析物浓度。 亚硝酸盐抑制由血红蛋白非酶促引起的干扰性染料形成。 优选地，试剂用于测量酮体例如β-羟基丁酸酯的干燥条。

    Diagnostics based on tetrazolium compounds
    8.
    发明授权
    Diagnostics based on tetrazolium compounds 有权

    公开(公告)号：US07144709B2

    公开(公告)日：2006-12-05

    申请号：US10990684

    申请日：2004-11-16

    申请人： Tianmei Ouyang ,  Yeung Siu Yu

    发明人： Tianmei Ouyang ,  Yeung Siu Yu

    IPC分类号： G01N33/53

    CPC分类号： C12Q1/32 ,  C12Q1/26 ,  C12Q1/54 ,  G01N33/525 ,  G01N33/64 ,  Y10S435/962 ,  Y10S435/97 ,  Y10T436/173076

    摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.

    Test strips for detecting the presence of a reduced cofactor in a sample and method for using the same
    9.
    发明授权
    Test strips for detecting the presence of a reduced cofactor in a sample and method for using the same 有权
    用于检测样品中还原辅因子的存在的测试条及其使用方法

    公开(公告)号：US06420128B1

    公开(公告)日：2002-07-16

    申请号：US09659938

    申请日：2000-09-12

    申请人： Tianmei Ouyang ,  Yeung Siu Yu

    发明人： Tianmei Ouyang ,  Yeung Siu Yu

    IPC分类号： C12Q154

    CPC分类号： G01N33/523 ,  Y10S435/975

    摘要： Test strips and methods for their use in the detection of an analyte in a sample are provided. The subject test strips are characterized by at least including a water soluble tetrazolium salt on a surface of a positively charged substrate. In many embodiments, the water soluble tetrazolium salt is present as part of an analyte oxidizing signal producing system, which system includes one or more of the following additional components: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; an electron transfer agent; and an enzyme cofactor. Also provided are systems and kits incorporating the subject test strips. The subject test strips, systems and kits find use in the measurement of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof.

    摘要翻译： 提供了用于检测样品中分析物的测试条和方法。 主题测试条的特征在于至少在带正电的基底的表面上包含水溶性四唑鎓盐。 在许多实施方案中，水溶性四唑盐作为分析物氧化信号产生系统的一部分存在，该系统包括一种或多种以下附加组分：分析物氧化酶，例如分析物脱氢酶或分析物氧化酶; 电子转移剂; 和酶辅因子。 还提供了包含主题测试条的系统和试剂盒。 主题测试条，系统和试剂盒可用于测量样品中各种分析物，例如生理样品，例如血液或其部分。

    Electrochemical test strip for use in analyte determination
    10.
    发明授权
    Electrochemical test strip for use in analyte determination 有权
    用于分析物测定的电化学测试条

    公开(公告)号：US06716577B1

    公开(公告)日：2004-04-06

    申请号：US09497269

    申请日：2000-02-02

    申请人： Yeung Siu Yu ,  Mahesh Shah

    发明人： Yeung Siu Yu ,  Mahesh Shah

    IPC分类号： C12I168

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/001 ,  G01N33/5438

    摘要： Electrochemical test strips and methods for their use in the detection of an analyte in a physiological sample are provided. The subject test strips have a reaction zone defined by opposing metal electrodes separated by a thin spacer layer. The metal surface of at least one of the electrodes is modified by a homogenous surface modification layer made up of linear self-assembling molecules having a first sulfhydryl end group and a second sulfonate end group separated by a short chain alkyl linking group, where 2-mercaptoethane sulfonic acid or a salt thereof is preferred in certain embodiments. The subject electrochemical test strips find application in the detection of a wide variety of analytes, and are particularly suited for use the detection of gluose.

    摘要翻译： 提供电化学测试条和它们用于检测生理样品中分析物的方法。 主题测试条具有由相对的金属电极限定的反应区，该间隔层由薄的间隔层隔开。 至少一个电极的金属表面被由具有第一巯基端基和由短链烷基连接基团分开的第二磺酸根端基的线性自组装分子组成的均匀表面改性层改性，其中2- 巯基乙磺酸或其盐在某些实施方案中是优选的。 主题电化学测试条可用于检测各种各样的分析物，特别适用于检测葡萄糖。