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公开(公告)号：US20080020386A1
公开(公告)日：2008-01-24
申请号：US11692565
申请日：2007-03-28
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172
摘要： A method for determining the human leukocyte antigen (HLA) genotype of a nucleic acid sample, comprises: contacting a nucleic acid sample with at least one nucleic acid primer set and subjecting the mixture to a nucleic acid amplification reaction; determining the size of any amplification products produced in the amplification reaction; and correlating the presence and/or absence of specific amplification products with the presence and/or absence of specific sequence polymorphisms in the nucleic acid sample. At least one of the primer sets is a multi-specific primer set comprising at least one sequence-specific forward primer and at least one sequence-specific reverse primer and being adapted to amplify two or more specific target sequences in the nucleic acid sample. Each of the specific target sequences comprises a sequence polymorphism that is known to be associated with an HLA allele and which may be present in the nucleic acid sample to be genotyped.
摘要翻译： 用于确定核酸样品的人白细胞抗原（HLA）基因型的方法包括：使核酸样品与至少一个核酸引物组接触，并使混合物进行核酸扩增反应; 确定在扩增反应中产生的任何扩增产物的大小; 并将特异性扩增产物的存在和/或不存在与核酸样品中特异性序列多态性的存在和/或不存在相关联。 引物组中的至少一个是包含至少一个序列特异性正向引物和至少一个序列特异性反向引物并适于扩增核酸样品中两个或多个特异性靶序列的多特异性引物组。 每个特定靶序列包含已知与HLA等位基因相关并且可存在于待基因型的核酸样品中的序列多态性。
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公开(公告)号：US5180237A
公开(公告)日：1993-01-19
申请号：US902711
申请日：1992-06-23
申请人： Yen-Chin Chen
发明人： Yen-Chin Chen
CPC分类号： B41J5/10 , G06F3/0202 , H01H2223/002 , H01H2229/028 , H01H2229/034 , H01H2231/044 , H01H2239/01
摘要： A keyboard housing includes a key plate with a plurality of actuator keys mounted on an upper face and a waterproof chamber mounted on a lower face thereof and a device to retain the actuator keys in a normally unpressed position. A circuit board is disposed in the waterproof chamber. A switch contact assembly mounted on the lower face of the key plate which includes a first membrane having a first conductive pattern, a second membrane having a second conductive pattern and a partition membrane disposed between the first and second membranes to separate the first conductive pattern from the second conductive pattern in a normal open switch. The partition membrane includes a hole device which permits a portion of the first conductive pattern to engage with a portion of the second conductive pattern when the first membrane is pressed by one of the actuator keys, thereby generating a signal corresponding to the pressing key. The periphery of the first membrane is adhesively connected to a face of the partition membrane, and the periphery of the second membrane is adhesively connected to the other face of the partition membrane. The conductive patterns of the first and second membranes are electrically connected to the circuit board.
摘要翻译： 键盘壳体包括具有安装在上表面上的多个致动器键和安装在其下表面上的防水室的键盘和用于将致动器键保持在正常未压紧位置的装置。 电路板设置在防水室中。 一种开关接触组件，其安装在所述键板的下表面上，所述开关接触组件包括具有第一导电图案的第一膜，具有第二导电图案的第二膜和设置在所述第一和第二膜之间的分隔膜，以将所述第一导电图案与 在正常打开开关中的第二导电图案。 分隔膜包括孔装置，当第一膜被其中一个致动器键按压时，允许第一导电图案的一部分与第二导电图案的一部分接合，从而产生对应于按压键的信号。 第一膜的周边与分隔膜的表面粘合地连接，第二膜的周边与分隔膜的另一面粘接。 第一和第二膜的导电图案电连接到电路板。
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3.发明申请公开(公告)号：US20150244350A1
公开(公告)日：2015-08-27
申请号：US14449161
申请日：2014-08-01
申请人： Yu-Chung Wang , Yen-Chin Chen
发明人： Yu-Chung Wang , Yen-Chin Chen
CPC分类号： H03K3/012 , H03K5/01 , H03K5/15013 , H03K2005/00286
摘要： A multi-phase signal generator and a multi-phase signal generating method thereof. The multi-phase signal generator includes a signal generator, a first comparator, a second comparator and a logic operation circuit. The signal generator generates a periodic signal. The first comparator receives the periodic signal and respectively compares the periodic signal with a first reference voltage and a second reference voltage to generate a first output signal. The second comparator receives the periodic signal and compares the periodic signal with a first threshold voltage to generate a second output signal. The logic operation circuit performs logic operations on the first output signal and the second output signal so as to generate a plurality of first phase output signals.
摘要翻译： 一种多相信号发生器及其多相信号发生方法。 多相信号发生器包括信号发生器，第一比较器，第二比较器和逻辑运算电路。 信号发生器产生周期信号。 第一比较器接收周期信号，并分别比较周期信号与第一参考电压和第二参考电压以产生第一输出信号。 第二比较器接收周期信号并将周期信号与第一阈值电压进行比较以产生第二输出信号。 逻辑运算电路对第一输出信号和第二输出信号执行逻辑运算，以产生多个第一相输出信号。
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公开(公告)号：US09257967B2
公开(公告)日：2016-02-09
申请号：US14449161
申请日：2014-08-01
申请人： Yu-Chung Wang , Yen-Chin Chen
发明人： Yu-Chung Wang , Yen-Chin Chen
摘要： A multi-phase signal generator and a multi-phase signal generating method thereof. The multi-phase signal generator includes a signal generator, a first comparator, a second comparator and a logic operation circuit. The signal generator generates a periodic signal. The first comparator receives the periodic signal and respectively compares the periodic signal with a first reference voltage and a second reference voltage to generate a first output signal. The second comparator receives the periodic signal and compares the periodic signal with a first threshold voltage to generate a second output signal. The logic operation circuit performs logic operations on the first output signal and the second output signal so as to generate a plurality of first phase output signals.
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公开(公告)号：USD341824S
公开(公告)日：1993-11-30
申请号：US854063
申请日：1992-03-19
申请人： Yen-Chin Chen
设计人： Yen-Chin Chen
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