公开(公告)号：US20060240006A1

公开(公告)日：2006-10-26

申请号：US11111098

申请日：2005-04-20

申请人： Chishih Chu ,  Li-Te Chin ,  Shu-Ching Hsu

发明人： Chishih Chu ,  Li-Te Chin ,  Shu-Ching Hsu

IPC分类号： G01N33/567 ,  A61K39/395 ,  C07K16/28

CPC分类号： C07K16/2818 ,  C07K2317/21

摘要： In order to provide necessary information for the production of complete human monoclonal antibodies capable of human CD152 (CTLA-4) binding, the primary structures of heavy and light chains have been elucidated. The novel amino acid sequence of identified heavy and light chains are derived from VH3 and Vλ germline genes, respectively. Antibodies comprising such novel structures cause specific binding to soluble recombinant human CD152 as well as to activated human peripheral T cells, where the expression of CD152 has been elevated.

公开(公告)号：US20130267688A1

公开(公告)日：2013-10-10

申请号：US13327752

申请日：2012-02-21

申请人： Li-Te Chin ,  Chishih Chu ,  Shu Ching Hsu

发明人： Li-Te Chin ,  Chishih Chu ,  Shu Ching Hsu

IPC分类号： C07K16/28

CPC分类号： C07K16/2818 ,  C07K2317/21

摘要： In order to provide necessary information for the production of complete human monoclonal antibodies capable of human CD152 (CTLA-4) binding, the primary structures of heavy and light chains have been elucidated. The novel amino acid sequence of identified heavy and light chains are derived from VH3 and Vλ germline genes, respectively. Antibodies comprising such novel structures cause specific binding to soluble recombinant human CD152 as well as to activated human peripheral T cells, where the expression of CD152 has been elevated.

摘要翻译： 为了提供生产能够进行人CD152（CTLA-4）结合的完整人单克隆抗体的必要信息，已经阐明了重链和轻链的一级结构。 鉴定的重链和轻链的新氨基酸序列分别衍生自VH3和Vlambda种系基因。 包含这种新型结构的抗体引起与可溶性重组人CD152的特异性结合以及CD152的表达升高的活化的人外周T细胞。

公开(公告)号：US06207167B1

公开(公告)日：2001-03-27

申请号：US09457072

申请日：1999-12-09

申请人： Jonathan T. Ou ,  Chishih Chu

发明人： Jonathan T. Ou ,  Chishih Chu

IPC分类号： A61K3902

CPC分类号： A61K39/015 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/53

摘要： A vaccine strain OU5758 is provided which includes an attenuated immunogenic plasmidless Salmonella enterica serovar-Typhimurium strain OU5046 harboring a recombinant plasmid pOU1500 for immunizing a chicken against leucocytozoonosis. The strain OU5046 is adapted to remain substantially avirulent, survive in vivo for at least a number of days, colonize within the chicken's liver, spleen, ovaries, or other internal organs, and express foreign antigens. The recombinant plasmid pOU1500 contains a gene encoding the circumsporozoite protein of the sporozoite of the chicken leucocytozoonosis parasite Leucocytozoon caulleryi. The vaccine strain OU5758 containing pOU1500 is capable of expressing circumsporozoite protein of L. caulleryi sporozoite, inducing anti-L. caulleryi immunity in chickens when ingested, reducing the frequency and severity of the disease's occurrence, and thereby enabling the chicken to maintain its normal egg production.

摘要翻译： 提供疫苗株OU5758，其包含减毒的免疫原性无质粒沙门氏菌沙门氏菌血清型 - 鼠伤寒沙门氏菌菌株OU5046，其携带重组质粒pOU1500，用于免疫鸡免于白细胞异常。 菌株OU5046适于保持基本无毒，在体内存活至少数天，在鸡的肝脏，脾脏，卵巢或其他内部器官内定殖并表达外源抗原。 重组质粒pOU1500含有编码鸡白细胞生成素寄生虫白细胞胞质的子孢子的周缘子孢子蛋白的基因。 含有pOU1500的疫苗株OU5758能够表达L. caulleryi子孢子的环子孢子蛋白，诱导抗L。 在摄入鸡时，鸡的免疫力下降，降低疾病发生的频率和严重程度，从而使鸡能够保持其正常的蛋产量。