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公开(公告)号：US4748129A
公开(公告)日：1988-05-31
申请号：US645458
申请日：1984-08-28
IPC分类号： G01N33/52 , A61K39/00 , G01N33/53 , G01N33/533 , G01N33/536 , G01N33/542 , G01N33/554 , G01N33/555 , G01N33/58 , G01N33/80
CPC分类号： G01N33/555 , G01N33/533 , G01N33/542 , G01N33/80 , Y10S436/80 , Y10S436/805 , Y10S436/807 , Y10S436/829
摘要： A method is provided for determining the presence in a sample of a member of a specific binding pair ("sbp member") consisting of ligand and its homologous receptor. The sample is combined in an aqueous medium with (1) a complementary sbp member wherein at least the sbp member or the complementary sbp member is bound to the surface of a cell and (2) a fuorescent agent capable of being incorporated into the cell. The presence of the sbp member is indicated by a change in fluorescence of the unseparated cell suspension as a result of agglutination of the cells.The present invention has particular application to blood typing, for example, for the determination of the presence of blood group antigens A, B, AB, O, and D (Rh.sub.o) and antibodies to such antigens.
摘要翻译： 提供了一种用于确定由配体及其同源受体组成的特异性结合对（“sbp成员”）的成员的样品中存在的方法。 样品在水性介质中与（1）互补sbp构件组合，其中至少sbp构件或互补sbp构件与细胞表面结合，和（2）能够结合到细胞中的荧光剂。 由于细胞凝集，未分离的细胞悬浮液的荧光变化表示sbp成员的存在。 本发明特别适用于血型分型，例如用于测定血型抗原A，B，AB，O和D（Rho）及其抗体的存在。
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公开(公告)号：US4857455A
公开(公告)日：1989-08-15
申请号：US444658
申请日：1982-11-26
申请人： Pyare L. Khanna , Chiu C. Chang , Edwin F. Ullman
发明人： Pyare L. Khanna , Chiu C. Chang , Edwin F. Ullman
IPC分类号： G01N33/50 , C07D311/06 , C07D311/22 , C07D311/86 , C07D493/10 , C07H21/00 , C12Q1/28 , G01N33/535
CPC分类号： C07H21/00 , C12Q1/28 , C12Q2326/00 , Y10S435/81 , Y10S435/968
摘要： Novel fluorogenic peroxidase substrates are provided employing novel water soluble activated capped aromatic fluorescent compounds for use as peroxidase substrates. The novel compounds can be used in a wide variety of ways for determination of peroxides, peroxidase, and in assays for analytes employing peroxidase as a label on a member of a specific binding pair.
摘要翻译： 提供使用新型水溶性活性封端芳香族荧光化合物作为过氧化物酶底物的新型荧光过氧化物酶底物。 新型化合物可以以多种方式用于测定过氧化物，过氧化物酶和在使用过氧化物酶作为特异性结合对成员上的标记的分析物的测定中。
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公开(公告)号：US5416214A
公开(公告)日：1995-05-16
申请号：US219323
申请日：1994-03-29
IPC分类号： C07C251/30 , C07C323/41 , C07F5/02 , C09B57/00 , C07D519/00
CPC分类号： C09B57/007 , C07C251/30 , C07C323/41 , C07F5/025
摘要： Novel squaraine dyes and compositions of matter containing such dyes are disclosed. The novel squaraine dyes have an absorption maximum greater than 600 nanometers and are particularly useful in conjunction with a helium/neon (He/Ne) laser. Some of the squaraine dyes are hydrophilic and are therefore water soluble or water compatible and others of the squaraine dyes are lipophilic.
摘要翻译： 公开了新型方酸菁染料和含有这些染料的物质组合物。 新型方酸染料的吸收最大值大于600纳米，特别适用于氦/氖（He / Ne）激光器。 一些方酸菁染料是亲水性的，因此是水溶性的或水相容的，而其他方酸菁染料是亲脂性的。
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公开(公告)号：US5437983A
公开(公告)日：1995-08-01
申请号：US13116
申请日：1993-02-01
IPC分类号： G01N33/537 , G01N33/538 , G01N33/543 , G01N33/546
CPC分类号： G01N33/538 , G01N33/54313 , Y10S436/824
摘要： A method for carrying out a binding assay is described wherein a member of a specific binding pair (sbp) and a sample are combined with a matrix of non-porous beads in a liquid medium under conditions such that the beads bind to the sbp member. The liquid medium is removed from the beads by aspiration using an aspiration tube having one or more orifices each of a diameter smaller than the minimum diameter of the smallest bead thereby allowing removal of the liquid medium while prohibiting aspiration of the beads.
摘要翻译： 描述了一种用于进行结合测定的方法，其中将特定结合对（sbp）和样品的成员与液体培养基中的无孔珠粒基质结合，使得珠子与sbp构件结合。 通过使用具有一个或多个直径小于最小珠的最小直径的孔的抽吸管通过抽吸将液体培养基从珠粒中除去，从而允许除去液体培养基，同时禁止吸入珠粒。
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公开(公告)号：US5039818A
公开(公告)日：1991-08-13
申请号：US300996
申请日：1989-01-23
申请人： John Pease , Thomas L. Tarnowski , Donald Berger , Chiu C. Chang
发明人： John Pease , Thomas L. Tarnowski , Donald Berger , Chiu C. Chang
IPC分类号： C07C251/30 , C07C323/41 , C07F5/02 , C09B57/00
CPC分类号： C09B57/007 , C07C251/30 , C07C323/41 , C07F5/025
摘要： Squaraine dyes and compositions of matter containing such dyes are disclosed. The squaraine dyes have an absorption maximum greater than 600 nanometers and are particularly useful in conjunction with a helium/neon (He/Ne) laser. Some of the squaraine dyes are hydrophilic and are therefore water soluble or water compatible and others of the squaraine dyes are lipophilic.
摘要翻译： 披露了方酸菁染料和含有这些染料的物质组合物。 方酸染料的吸收最大值大于600纳米，特别适用于氦/氖（He / Ne）激光器。 一些方酸菁染料是亲水性的，因此是水溶性的或水相容的，而其他方酸菁染料是亲脂性的。
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公开(公告)号：US4830786A
公开(公告)日：1989-05-16
申请号：US834168
申请日：1986-02-27
IPC分类号： C07C251/30 , C07C323/41 , C07F5/02 , C09B57/00
CPC分类号： C07F5/025 , C07C251/30 , C07C323/41 , C09B57/007
摘要： Squaraine dyes and compositions of matter containing such dyes are disclosed. The squaraine dyes have an absorption maximum greater than 600 nanometers and are particulary useful in conjunction with a helium/neon (He/Ne) laser. Some of the squaraine dyes are hydrophilic and are therefore water soluble or water compatible and others of the squaraine dyes are lipophilic.
摘要翻译： 披露了方酸菁染料和含有这些染料的物质组合物。 方酸菁染料的吸收最大值大于600纳米，特别适用于氦/氖（He / Ne）激光器。 一些方酸菁染料是亲水性的，因此是水溶性的或水相容的，而其他方酸菁染料是亲脂性的。
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公开(公告)号：US5329019A
公开(公告)日：1994-07-12
申请号：US874659
申请日：1992-04-27
IPC分类号： C07C251/30 , C07C323/41 , C07F5/02 , C09B57/00 , C07D403/08
CPC分类号： C09B57/007 , C07C251/30 , C07C323/41 , C07F5/025
摘要： Novel squaraine dyes and compositions of matter containing such dyes are disclosed . The novel squaraine dyes have an absorption maximum greater than 600 nanometers and are particularly useful in conjunction with a helium/neon (He/Ne) laser. Some of the squaraine dyes are hydrophilic and are therefore water soluble or water compatible and others of the squaraine dyes are lipophilic.
摘要翻译： 公开了新型方酸菁染料和含有这些染料的物质组合物。 新型方酸染料的吸收最大值大于600纳米，特别适用于氦/氖（He / Ne）激光器。 一些方酸菁染料是亲水性的，因此是水溶性的或水相容的，而其他方酸菁染料是亲脂性的。
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公开(公告)号：US5310922A
公开(公告)日：1994-05-10
申请号：US714964
申请日：1991-06-13
IPC分类号： C07C251/30 , C07C323/41 , C07F5/02 , C09B57/00 , C07D417/08
CPC分类号： C09B57/007 , C07C251/30 , C07C323/41 , C07F5/025
摘要： Novel squaraine dyes and compositions of matter containing such dyes are disclosed. The novel squaraine dyes have an absorption maximum greater than 600 nanometers and are particularly useful in conjunction with a helium/neon (He/Ne) laser. Some of the squaraine dyes are hydrophilic and are therefore water soluble or water compatible and others of the squaraine dyes are lipophilic.
摘要翻译： 公开了新型方酸菁染料和含有这些染料的物质组合物。 新型方酸染料的吸收最大值大于600纳米，特别适用于氦/氖（He / Ne）激光器。 一些方酸菁染料是亲水性的，因此是水溶性的或水相容的，而其他方酸菁染料是亲脂性的。
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