METHODS FOR SEPARATING CASEIN FROM SOLUBLE PROTEINS IN A COMPOSITION
    1.
    发明申请
    METHODS FOR SEPARATING CASEIN FROM SOLUBLE PROTEINS IN A COMPOSITION 有权
    在组合物中分离可溶性蛋白质的方法

    公开(公告)号:US20090169699A1

    公开(公告)日:2009-07-02

    申请号:US12034256

    申请日:2008-02-20

    IPC分类号: A23C7/04

    摘要: Methods for separating casein from soluble proteins in a composition. The method includes: adding a phosphate solution to the composition, mixing to form a mixture, freezing the mixture to obtain a frozen mixture, thawing the frozen mixture to obtain casein-containing aggregates in the mixture, and then centrifuging the mixture to obtain a supernatant traction and a casein-precipitate fraction. The supernatant fraction contains the soluble protein, which may be isolated by subjecting the supernatant fraction to a purification process. The method can be applied to purify a recombinant protein from the milk of a transgenic mammal.

    摘要翻译: 将酪蛋白与可溶性蛋白质分离在组合物中的方法。 该方法包括:向组合物中加入磷酸盐溶液,混合形成混合物,冷冻混合物以获得冷冻混合物,解冻冷冻混合物,得到混合物中含酪蛋白的聚集体,然后离心分离得到上清液 牵引和酪蛋白沉淀部分。 上清液部分含有可溶性蛋白质,其可以通过使上清液部分进行纯化过程来分离。 该方法可用于从转基因哺乳动物的乳中纯化重组蛋白。

    Method for expressing multiple recombinant proteins in milk of transgenic non-human mammals
    4.
    发明申请
    Method for expressing multiple recombinant proteins in milk of transgenic non-human mammals 有权
    用于在转基因非人哺乳动物的乳中表达多种重组蛋白的方法

    公开(公告)号:US20050114911A1

    公开(公告)日:2005-05-26

    申请号:US10790720

    申请日:2004-03-03

    IPC分类号: A01K67/027

    摘要: The present invention discloses a method for expressing multiple recombinant proteins in transgenic non-human mammalian milk, characterized in which human clotting factor IX gene and porcine lactoferrin gene are transferred into the mammal by gene injection and embryonic implantation to obtain expression in the milk of transgenic mammal and its filial generation. The method of this invention can maintain the stable expression of multiple recombinant proteins in the transgenic mammal during lactation and stable expression amount proximate to that of the first generation in the offsprings of the transgenic non-human mammal.

    摘要翻译: 本发明公开了一种在转基因非人哺乳动物乳中表达多种重组蛋白的方法,其特征在于通过基因注射和胚胎移植将人凝血因子IX基因和猪乳铁蛋白基因转移到哺乳动物中,以获得转基因乳中的表达 哺乳动物及其子代。 本发明的方法可以在哺乳期间保持转基因哺乳动物中多种重组蛋白的稳定表达,并且在转基因非人哺乳动物的后代中可以保持接近于第一代的稳定表达量。