公开(公告)号：US07038035B1

公开(公告)日：2006-05-02

申请号：US09719533

申请日：1998-06-19

申请人： Chong Jin Oon ,  Gek Keow Lim ,  Ai Lin Leong ,  Yi Zhao ,  Wei Ning Chen

发明人： Chong Jin Oon ,  Gek Keow Lim ,  Ai Lin Leong ,  Yi Zhao ,  Wei Ning Chen

IPC分类号： C07H21/04 ,  C12N7/00 ,  C12N7/01 ,  C12N15/09 ,  C12N15/51

CPC分类号： G01N33/5764 ,  A61K39/12 ,  A61K39/29 ,  C07K14/005 ,  C07K14/71 ,  C07K2319/00 ,  C07K2319/30 ,  C12N7/00 ,  C12N2730/10122 ,  C12N2730/10134 ,  C12Q1/706

摘要： This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-'Ś-145 Singapore Strain (Glycine to Arginine) which constituent viral genome is deposited under Accession Nos. P97121504, P97121505 and P97121506 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 145 of such polypeptide is an arginine rather than a glycine, and the purified peptide. This invention further provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. ID. No. 1, and the purified peptide. This invention also provides various methods using the disclosed isolated nucleic acids and polypeptides. This invention also provides various uses of the viral strain and its proteins.

摘要翻译： 本发明提供了一种分离的乙型肝炎病毒株，命名为人乙型肝炎病毒表面抗原-145新加坡菌株（Glycine to Arginine），其构成病毒基因组以保藏号P97121504，P97121505和P97121506与欧洲细胞集合 本发明还提供了编码多肽的分离核酸，其是乙型肝炎病毒株的突变型主要表面抗原，该多肽具有不同于主要的氨基酸序列的氨基酸序列 野生型乙型肝炎病毒的表面抗原是这种多肽的位置编号145的氨基酸是精氨酸而不是甘氨酸，和纯化的肽。 本发明还提供了编码肽的分离的核酸，其中所述肽由包含SEQ ID NO：5的核苷酸527至595的核酸分子编码。 ID。 1和纯化肽。 本发明还提供了使用公开的分离的核酸和多肽的各种方法。 本发明还提供了病毒株及其蛋白质的各种用途。

公开(公告)号：US07037682B2

公开(公告)日：2006-05-02

申请号：US10231405

申请日：2002-08-28

申请人： Chong Jin Oon ,  Wei Ning Chen ,  Gek Keow Lim ,  Ai Lin Leong

发明人： Chong Jin Oon ,  Wei Ning Chen ,  Gek Keow Lim ,  Ai Lin Leong

IPC分类号： C12P21/06 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/706 ,  C12N9/1252 ,  C12Q1/686 ,  C12Q2521/101

摘要： The present invention provides an in vitro activity assay for human hepatitis B virus (HBV) DNA polymerase, which comprises using, as the 5′ oligonucleotide in PCR amplification of HBV DNA polymerase from a sample, an oligonucleotide into which has been incorporated the SP6 viral polymerase promoter, directly transcribing and translating the PCR products in the presence of a radio-labelled agent and measuring the priming of the HBV DNA polymerase. The present invention also provides the use of such an assay to assay activity of various serum samples, to screen for inhibitors of the HBV DNA polymerase and to test and/or screen potential anti-HBV drugs for their ability to inhibit DNA priming activity of human HBV DNA polymerase.

公开(公告)号：US06593082B1

公开(公告)日：2003-07-15

申请号：US09674465

申请日：2001-06-18

申请人： Chong Jin Oon ,  Wei Ning Chen ,  Gek Keow Lim ,  Ai Lin Leong

发明人： Chong Jin Oon ,  Wei Ning Chen ,  Gek Keow Lim ,  Ai Lin Leong

IPC分类号： C12Q170

CPC分类号： C12Q1/706 ,  C12N9/1252 ,  C12Q2525/143

摘要： The present invention provides an in vitro activity assay for human hepatitis B virus (HBV) DNA polymerase, which comprises using, as the 5′ oligonucleotide in PCR amplification of HBV DNA polymerase from a sample, an oligonucleotide into which has been incorporated the SP6 viral polymerase promoter, directly transcribing and translating the PCR products in the presence of a radio-labelled agent and measuring the priming of the HBV DNA polymerase. The present invention also provides the use of such an assay to assay activity of various serum samples, to screen for inhibitors of the HBV DNA polymerase and to test and/or screen potential anti-HBV drugs for their ability to inhibit DNA priming activity of human HBV DNA polymerase.

公开(公告)号：US07105165B2

公开(公告)日：2006-09-12

申请号：US10761006

申请日：2004-01-20

申请人： Chong Jin Oon ,  Gek Keow Lim ,  Yi Zhao ,  Wei Ning Chen

发明人： Chong Jin Oon ,  Gek Keow Lim ,  Yi Zhao ,  Wei Ning Chen

IPC分类号： A39 ,  C12N7/01 ,  C12N7/02

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K2039/525 ,  C07K1/22 ,  C12N2730/10122

摘要： This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide. This invention also provides various methods of using the disclosed isolated nucleic acid and peptides.

摘要翻译： 本发明提供了一种分离的乙型肝炎病毒株，其命名为人乙型肝炎病毒表面抗原-'S'-133 Oon菌株（甲硫氨酸至苏氨酸），构成病毒基因组以保藏号P97121501，P97121502和P97121503保藏在欧洲集合 细胞培养在15日/ 12月12日。 本发明还提供了编码多肽的分离核酸，其是乙型肝炎病毒株的突变型主要表面抗原，该多肽具有不同于野生型主要表面抗原的氨基酸序列的氨基酸序列 因为该多肽的第133位氨基酸是苏氨酸，而不是甲硫氨酸。 本发明还提供了编码肽的分离的核酸，其中肽由包含SEQ ID NO：5的核苷酸527至595的核酸分子编码。 ID。 1和纯化肽。 本发明还提供使用所公开的分离的核酸和肽的各种方法。

公开(公告)号：US06787142B2

公开(公告)日：2004-09-07

申请号：US10209264

申请日：2002-07-31

申请人： Chong Jin Oon ,  Gek Keow Lim ,  Yi Zhao ,  Wei Ning Chen

发明人： Chong Jin Oon ,  Gek Keow Lim ,  Yi Zhao ,  Wei Ning Chen

IPC分类号： A61K3929

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K2039/525 ,  C07K1/22 ,  C12N2730/10122

摘要： This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th December 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide. This invention also provides various methods of using the disclosed isolated nucleic acid and peptides.

摘要翻译： 本发明提供了一种分离的乙型肝炎病毒株，其命名为人乙型肝炎病毒表面抗原-'S'-133 Oon菌株（甲硫氨酸至苏氨酸），构成病毒基因组以保藏号P97121501，P97121502和P97121503保藏在欧洲集合 细胞培养。本发明还提供编码多肽的分离核酸，其是乙型肝炎病毒株的突变型主要表面抗原，该多肽具有不同于氨基酸序列的氨基酸序列 的野生型乙型肝炎病毒的主要表面抗原，因为该多肽的第133位的氨基酸是苏氨酸而不是甲硫氨酸。 本发明还提供了编码肽的分离的核酸，其中肽由包含SEQ ID NO：5的核苷酸527至595的核酸分子编码。 ID。 1和纯化肽。 本发明还提供使用所公开的分离的核酸和肽的各种方法。

公开(公告)号：US06558675B1

公开(公告)日：2003-05-06

申请号：US09719528

申请日：2001-04-30

申请人： Chong Jin Oon ,  Gek Keow Lim ,  Yi Zhao ,  Wei Ning Chen

发明人： Chong Jin Oon ,  Gek Keow Lim ,  Yi Zhao ,  Wei Ning Chen

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2730/10122

摘要： An isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) of Cell. Also, an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than methionine.

摘要翻译： 一种分离的乙型肝炎病毒株，命名为人类乙型肝炎病毒表面抗原 - 细胞的“S'-133 Oon菌株（甲硫氨酸至苏氨酸）”。此外，编码多肽的分离的核酸是多肽的突变主要表面抗原 乙型肝炎病毒，这种多肽具有与野生型乙型肝炎病毒的主要表面抗原的氨基酸序列不同的氨基酸序列，因为该多肽的第133位的氨基酸是苏氨酸而不是甲硫氨酸。