公开(公告)号：US20240229093A1

公开(公告)日：2024-07-11

申请号：US18562047

申请日：2021-05-20

Applicant: Chr. Hansen A/S

Inventor： Katja Parschat ,  Stefan Jennewein

IPC: C12P19/26 ,  C12N1/16 ,  C12N1/20 ,  C12N9/10 ,  C12R1/07 ,  C12R1/185 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85

CPC classification number: C12P19/26 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1051 ,  C12N2500/34 ,  C12R2001/07 ,  C12R2001/185 ,  C12R2001/78 ,  C12R2001/84 ,  C12R2001/85 ,  C12Y204/0109

Abstract: Disclosed is a process for the production of a desired oligosaccharide, the process comprises providing a genetically engineered microbial cell which possesses a saccharide importer for the uptake of an intermediate oligosaccharide, and an enzyme being able to convert the intermediated oligosaccharide by transferring a monosaccharide moiety from a donor substrate to the intermediate oligosaccharide; cultivating the genetically engineered microbial cell in the presence of an intermediate oligosaccharide to generate the desired oligosaccharide; and retrieving the desired oligosaccharide.