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公开(公告)号：US06514773B1
公开(公告)日：2003-02-04
申请号：US08506268
申请日：1995-07-24
申请人： Christian Klein , Hans-Peter Josel , Ada Goerlach-Graw , Reinhold Hilpert , Florian Binder , Josef Ritter , Rudolf Zimmermann
发明人： Christian Klein , Hans-Peter Josel , Ada Goerlach-Graw , Reinhold Hilpert , Florian Binder , Josef Ritter , Rudolf Zimmermann
IPC分类号： G01N33544
CPC分类号： C07D451/02 , C07D489/02 , G01N1/02 , G01N33/558 , G01N2001/007 , G01N2001/028 , Y10S436/809 , Y10S436/81
摘要： Subject matter of the invention is a method for detecting surface contamination by an analyte by wiping the analyte off the surface with the aid of a wiping surface, eluting the analyte from the wiping surface with an eluant, and detecting the analyte in the eluate in an immunological detection reaction, characterized in that: a) the surface to be tested for the analyte is wiped with a wiping surface, b) the wiping surface is brought into contact with the planar surface of a capillary active, chromatographic test strip which has an eluant application zone at its one end and a target zone at its other end whereby contact is made in an area between these two zones, c) eluting liquid is applied onto the zone provided for this purpose, said liquid moving toward the target zone passes the contact site with the wiping surface as a consequence of capillary forces, whereby analyte is taken up by the eluant, and d) in the target zone, the analyte is measured in an immunological binding reaction. The method is particularly suitable for the detection of drugs on surfaces.
摘要翻译： 本发明的主题是通过擦拭表面擦拭分析物离开表面的方法，用分析物检测表面污染物的方法，用洗脱液从擦拭表面洗脱分析物，并用洗脱液检测洗脱液中的分析物 免疫学检测反应，其特征在于：a）待分析物的表面用擦拭表面擦拭，b）擦拭表面与具有洗脱剂的毛细管活性色谱测试条的平面接触 在其一端的应用区域和其另一端的目标区域，由此在这两个区域之间的区域中进行接触，c）将洗脱液施加到为此目的设置的区域上，朝向目标区域移动的所述液体通过接触 作为毛细管力的结果，由于分析物被目标区域中的洗脱液和d）吸收，所以在免疫结合反应中测量分析物。 方法特别适用于检测表面上的药物。
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2.发明授权公开(公告)号：US07026002B1
公开(公告)日：2006-04-11
申请号：US09594972
申请日：2000-06-15
IPC分类号： G01N33/53
CPC分类号： G01N33/558 , Y10S435/969 , Y10S435/97 , Y10S435/975 , Y10S436/81
摘要： The invention concerns an element for the determination of an analyte in a liquid by means of a specific binding reaction of two bioaffine binding partners containing in or on material which enables liquid transport between the zones. The element comprises a sample application zone; a detection zone located downstream thereof that is devoid of binding reagents and is the last zone on the element that allows liquid transport; a zone containing immobilized analyte or analyte analogue between the sample application zone and the detection zone; and impregnated conjugate that can be detached by liquid located in the sample application zone or upstream or downstream thereof composed of a bioaffine binding partner capable of a specific binding reaction with the analyte to be determined and a detectable label.
摘要翻译： 本发明涉及一种用于通过两种生物亚胺结合配偶体的特异性结合反应来测定液体中的分析物的元件，所述两种生物石蜡结合配对物质包含在能够在区域之间进行液体输送的材料中或材料上。 元件包括样品施加区域; 位于其下游的没有结合试剂的检测区，并且是允许液体输送的元件上的最后一个区域; 在样品施用区域和检测区域之间含有固定化分析物或分析物类似物的区域; 以及可以通过位于样品施用区域中的液体或其上游或下游由能够与待测定的分析物进行特异性结合反应的生物熔解结合对象组成的浸渍的共轭物和可检测标记物。
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公开(公告)号：US5556789A
公开(公告)日：1996-09-17
申请号：US270162
申请日：1994-07-01
申请人： Ada Goerlach-Graw , Reinhard Baer , Rolf Lerch
发明人： Ada Goerlach-Graw , Reinhard Baer , Rolf Lerch
CPC分类号： B01L3/5023 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/084 , Y10S436/807 , Y10S436/901
摘要： Device for the determination of analytes having a sample application point, several separate sample withdrawal zones that are each connected with the sample application point by one capillary transport path and that have several test elements for the individual determination of analytes wherein a retardation zone is provided on at least one of the transport paths. Material for the capillary transport of a liquid sample defines a sample application zone and a plurality of sample withdrawal zones connected to said application zone by a like plurality of transport paths. At least one of said paths has a retardation zone which assures that liquid applied to said application zone arrives simultaneously at said withdrawal zones, regardless of the lengths of the paths.
摘要翻译： 用于测定具有样品应用点的分析物的装置，几个单独的取样区，其各自的样品取出区各自通过一个毛细管输送路径与样品施加点连接，并且具有用于单独测定分析物的几个测试元件，其中延迟区设置在 至少一个传输路径。 用于液体样品的毛细管输送的材料限定样品施加区域和通过相同的多个输送路径连接到所述施加区域的多个取样区域。 所述路径中的至少一个具有延迟区域，其确保施加到所述应用区域的液体同时到达所述撤回区域，而不管路径的长度。
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公开(公告)号：US07267992B2
公开(公告)日：2007-09-11
申请号：US11348705
申请日：2006-02-07
IPC分类号： G01N33/53
CPC分类号： G01N33/558 , Y10S435/969 , Y10S435/97 , Y10S435/975 , Y10S436/81
摘要： A method is provided to determine the presence of an analyte in a sample. The method provides an element with a sample application zone, a detection zone, a zone containing immobilized analyte or analyte analogue, a conjugate that has a first bioaffine binding partner capable of a specific binding reaction with the analyte and a first detectable label, and a universal conjugate, which can be detached by liquid and comprises a second bioaffine binding partner and a visually detectable label. The second bioaffine binding partner is capable of a specific binding reaction with the first detectable label. The method also includes contacting the sample application zone with the sample, contacting the sample with the conjugate and the universal conjugate to react the analyte with the and the universal conjugate to form a detection complex, and determining the presence of the detection complex in the detection zone of the element.
摘要翻译： 提供了一种确定样品中分析物的存在的方法。 该方法提供了具有样品施用区域，检测区域，含有固定化分析物或分析物类似物的区域的元件，具有能够与分析物和第一可检测标记物进行特异性结合反应的第一生物亚烷结合配偶体的缀合物，以及 通用缀合物，其可以通过液体分离并且包含第二生物碱结合配偶体和视觉上可检测的标记。 第二生物石墨结合配偶体能够与第一可检测标记物进行特异性结合反应。 该方法还包括使样品施用区与样品接触，使样品与缀合物和通用缀合物接触以使分析物与通用缀合物反应以形成检测复合物，并确定检测复合物在检测中的存在 元素的区域。
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公开(公告)号：US20060128029A1
公开(公告)日：2006-06-15
申请号：US11348705
申请日：2006-02-07
IPC分类号： G01N33/558 , G01N33/551
CPC分类号： G01N33/558 , Y10S435/969 , Y10S435/97 , Y10S435/975 , Y10S436/81
摘要： A method is provided to determine the presence of an analyte in a sample. The method provides an element with a sample application zone, a detection zone, a zone containing immobilized analyte or analyte analogue, a conjugate that has a first bioaffine binding partner capable of a specific binding reaction with the analyte and a first detectable label, and a universal conjugate, which can be detached by liquid and comprises a second bioaffine binding partner and a visually detectable label. The second bioaffine binding partner is capable of a specific binding reaction with the first detectable label. The method also includes contacting the sample application zone with the sample, contacting the sample with the conjugate and the universal conjugate to react the analyte with the and the universal conjugate to form a detection complex, and determining the presence of the detection complex in the detection zone of the element.
摘要翻译： 提供了一种确定样品中分析物的存在的方法。 该方法提供了具有样品施用区域，检测区域，含有固定化分析物或分析物类似物的区域的元件，具有能够与分析物和第一可检测标记物进行特异性结合反应的第一生物亚烷结合配偶体的缀合物，以及 通用缀合物，其可以通过液体分离并且包含第二生物碱结合配偶体和视觉上可检测的标记。 第二生物石墨结合配偶体能够与第一可检测标记物进行特异性结合反应。 该方法还包括使样品施用区与样品接触，使样品与缀合物和通用缀合物接触以使分析物与通用缀合物反应以形成检测复合物，并确定检测复合物在检测中的存在 元素的区域。
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6.发明授权Analysis element and method for determination of an analyte in a liquid sample 失效用于测定液体样品中分析物的分析元素和方法
公开(公告)号：US5424220A
公开(公告)日：1995-06-13
申请号：US10985
申请日：1993-01-29
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/558 , Y10S435/805 , Y10S435/81 , Y10S435/967 , Y10S435/97 , Y10S435/973 , Y10S436/805 , Y10S436/807 , Y10S436/81
摘要： The invention addresses an analysis element for the determination of an analyte according to the principle of a heterogeneous immunoassay. Said analysis element is made of a chromatographic porous carrier material and has a reaction zone, at least two detection zones and absorptive zones following said detection zones. The reaction zone contains analyte-specific and labelled binding partners which are present in a number of soluble compartments which are close together, but nevertheless spatially separated. The detection zones are adjacent to the reaction zones and contain an immobilized binding reagent for one of the binding partners present in the reaction zone. At least one detection zone contains a binding reagent for the analyte-specific unlabelled binding partner.
摘要翻译： 本发明解决了根据异源免疫测定原理确定分析物的分析元件。 所述分析元件由色谱多孔载体材料制成，并且具有反应区域，在所述检测区域之后具有至少两个检测区域和吸收区域。 反应区含有分析物特异性和标记的结合配偶体，它们存在于多个可靠的隔室中，它们靠近在一起，但仍然在空间上分离。 检测区域与反应区域相邻并且含有用于存在于反应区域中的一个结合配偶体的固定化结合试剂。 至少一个检测区域包含用于分析物特异性未标记结合配偶体的结合试剂。
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