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    ENCAPSULATED REAGENTS AND METHODS OF USE
    1.
    发明申请
    ENCAPSULATED REAGENTS AND METHODS OF USE 有权
    包封试剂和使用方法

    公开(公告)号：US20140335518A1

    公开(公告)日：2014-11-13

    申请号：US14336500

    申请日：2014-07-21

    申请人： Lee H. Angros

    发明人： Lee H. Angros

    IPC分类号： C12Q1/68

    CPC分类号： G01N1/30 ,  B01L3/52 ,  B01L3/523 ,  B01L2200/0673 ,  B01L2300/18 ,  B01L2300/1861 ,  C12Q1/68 ,  C12Q1/6841 ,  G01N35/1002 ,  G01N2035/00237 ,  Y10S435/962 ,  Y10S435/969

    摘要： The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.

    摘要翻译： 本发明考虑使用封装的水性和非水性试剂，溶液和溶剂以及它们在实验室程序中的用途。 这些包封的水性或非水性试剂，溶液和溶剂可以完全包含或包封在微胶囊或纳米胶囊中，可以加入水性或非水性载体溶液或生物或非生物学的医学和研究实验室检测所需的液体 标本。

    Magnetic-nanoparticle conjugates and methods of use
    2.
    发明申请
    Magnetic-nanoparticle conjugates and methods of use 审中-公开
    磁性纳米颗粒共轭物和使用方法

    公开(公告)号：US20140303022A1

    公开(公告)日：2014-10-09

    申请号：US14256804

    申请日：2014-04-18

    申请人： The General Hospital Corporation

    发明人： Lee Josephson ,  Ralph Weissleder ,  J. Manuel Perez

    IPC分类号： G01N33/543 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/68

    CPC分类号： G01N33/54346 ,  A61K49/1851 ,  B82Y5/00 ,  C07F15/02 ,  C07H21/02 ,  C07K1/22 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/6816 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/577 ,  Y10S435/969 ,  Y10S977/773 ,  Y10S977/918 ,  Y10S977/92 ,  Y10T436/143333

    摘要： The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.

    摘要翻译： 本发明提供结合部分 - 纳米颗粒共轭物的新组合物，这些缀合物的聚集体，以及使用这些共轭物和聚集体的新方法。 这些共轭物中的纳米颗粒可以是单分散或多分散的磁性金属氧化物。 结合部分可以是例如寡核苷酸，多肽或多糖。 寡核苷酸序列与非聚合物表面官能化金属氧化物或与金属氧化物相关的官能化聚合物连接。 该新型组合物可用于体外检测靶分子（例如核酸和蛋白质）或作为磁共振（MR）造影剂检测活体中靶分子的测定。

    Method for identifying differentially expressed proteins
    4.
    发明授权
    Method for identifying differentially expressed proteins 有权
    识别差异表达蛋白质的方法

    公开(公告)号：US08071322B2

    公开(公告)日：2011-12-06

    申请号：US10705109

    申请日：2003-11-10

    申请人： Dongxiao Zhang

    发明人： Dongxiao Zhang

    IPC分类号： G01N33/53 ,  G01N33/554 ,  G01N33/574

    CPC分类号： G01N33/57484 ,  G01N33/5011 ,  Y10S435/969 ,  Y10S436/813

    摘要： The invention provides a method of identifying an antigen that is present in different amounts in two different samples. In general, the methods involve generating a first and second distinguishably labeled population of antibodies that reactive to the two samples, contacting the first and second labeled populations of antibodies with a plurality of antigens; and identifying any resultant antigens that are differentially bound by the first and second populations of antibodies. The antigens may be on the surface of cells e.g., animal cells, or on a solid support. Once identified, the nucleic acid encoding an antigen of interest may be identified and sequenced to reveal the identity of the antigen of interest. Kits for performing the methods are also provided. The methods find most use in medical and research applications, in particular, for identifying cell surface targets for immunotherapy and drug discovery.

    摘要翻译： 本发明提供了鉴定在两个不同样品中以不同量存在的抗原的方法。 通常，所述方法包括产生对两个样品有反应性的第一和第二可辨别地标记的抗体群，使抗体的第一和第二标记群体与多个抗原接触; 并鉴定由第一和第二群抗体差异结合的任何所得抗原。 抗原可以在细胞的表面上，例如动物细胞或固体支持物上。 一旦被鉴定，可以鉴定和测序编码感兴趣的抗原的核酸，以揭示感兴趣的抗原的身份。 还提供了用于执行方法的工具包。 该方法最适用于医学和研究应用，特别是用于鉴定用于免疫治疗和药物发现的细胞表面靶标。

    Multiple immunochemistry assays on an element
    6.
    发明授权
    Multiple immunochemistry assays on an element 有权
    元素上的多重免疫化学测定

    公开(公告)号：US07816090B2

    公开(公告)日：2010-10-19

    申请号：US11621797

    申请日：2007-01-10

    申请人： Merrit N. Jacobs

    发明人： Merrit N. Jacobs

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54386 ,  Y10S435/96 ,  Y10S435/969 ,  Y10S435/971 ,  Y10S435/973

    摘要： A method of analyzing a sample for one or more analytes of interest includes: providing an element having a base layer; a layer containing streptavidin; a spreading layer, wherein the streptavidin may or may not be in the spreading layer; providing immunocomponents and labeled immunoreactants which may be in the spreading layer or may be combined with the sample; dispensing the sample, and optionally the immunocomponents and a labeled immunoreactant onto the spreading layer at three or more areas, wherein each center of the three or more areas is substantially equidistance to the center formed by the three or more areas, and wherein each area contacts an adjacent area such that wash fluid flow in any direction will contact sample; washing the sample and label by directing the wash fluid at the center formed by the three or more areas, whereby the wash fluid equally flows over each of the three or more areas; and taking a measurement at each three or more areas to determine the presence or concentration of the one or more analyte. In a preferred embodiment, the element is a dry slide used on a diagnostic analyzer.

    摘要翻译： 分析一种或多种感兴趣分析物的样品的方法包括：提供具有基础层的元件; 含有链霉亲和素的层; 扩散层，其中链霉抗生物素蛋白可以或不在扩散层中; 提供可以在扩散层中或可以与样品组合的免疫组分和标记的免疫反应物; 将样品和任选的免疫组分和标记的免疫反应物分配到三个或更多个区域的扩散层上，其中三个或更多个区域的每个中心与由三个或更多个区域形成的中心基本上等距，并且其中每个区域接触 相邻区域使得洗涤流体沿任何方向流动将接触样品; 通过将洗涤流体引导到由三个或更多个区域形成的中心处洗涤样品和标签，由此洗涤流体均匀地流过三个或更多个区域中的每一个; 并在每个三个或更多个区域进行测量以确定一种或多种分析物的存在或浓度。 在优选实施例中，该元件是用于诊断分析器的干燥载玻片。

    Native analyte as a reference in lateral flow assays
    7.
    发明申请
    Native analyte as a reference in lateral flow assays 有权
    天然分析物作为侧流测定中的参考

    公开(公告)号：US20070172963A1

    公开(公告)日：2007-07-26

    申请号：US10557300

    申请日：2004-06-03

    申请人： Gary Krauth ,  David Ledden

    发明人： Gary Krauth ,  David Ledden

    IPC分类号： G01N33/558

    CPC分类号： G01N33/558 ,  G01N33/56911 ,  G01N33/56927 ,  G01N33/56988 ,  G01N33/571 ,  G01N33/6854 ,  G01N33/6887 ,  G01N33/689 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/81

    摘要： This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.

    摘要翻译： 本发明涉及用于检测液体测试样品中分析物的存在的侧向流动测定。 横向流量测定表示准确地并且以高保真度检测液体样品中目标分析物的存在或不存在的能力的改进，部分地通过包含允许检测的固定的不扩散分析物的参考区域 干扰分析物与标记的捕获试剂的相互作用和结合的任何因素。 在液体测试样品中溶液中游离的分析物与其互补的标记试剂之间的相互作用和结合的任何影响将与参考区域中的固定化分析物与标记试剂的结合并行遇到，因为它通过参考文献扩散 地区。 在一个实施方案中，本发明的侧流测定是基于尿液的人绒毛膜促性腺激素（hCG）测定。

    Carriers coated with polysaccharides, their preparation and use
    8.
    发明授权
    Carriers coated with polysaccharides, their preparation and use 有权
    载体涂多糖，其制备和用途

    公开(公告)号：US07179660B1

    公开(公告)日：2007-02-20

    申请号：US10220623

    申请日：2000-03-06

    申请人： Hrair Kirakossian ,  John S. Pease ,  Carsten Schelp ,  Marcel R. Pirio ,  Uwe Stöhr ,  Andreas Wiegand

    发明人： Hrair Kirakossian ,  John S. Pease ,  Carsten Schelp ,  Marcel R. Pirio ,  Uwe Stöhr ,  Andreas Wiegand

    IPC分类号： G01N33/548

    CPC分类号： G01N33/548 ,  Y10S435/969 ,  Y10S436/827 ,  Y10T428/2991

    摘要： A polysaccharide coated carrier having a coating of at least two successive layers of polysaccharide. The first polysaccharide layer spontaneously associates with a second polysaccharide layer and, optionally, the carrier. Each successive layer of polysaccharide spontaneously associates with a preceding layer. Spontaneous association occurs due to the presence of oppositely charged functional groups on each layer of polysaccharide or due to a spontaneous reaction between the functional groups the layers. The carrier may be any surface such as a tube, microtitration plate, bead, particle or the like and is suitable for use in diagnostic or therapeutic methods.

    摘要翻译： 具有至少两层连续多层多糖的涂层的多糖涂覆的载体。 第一多糖层自发地与第二多糖层和任选的载体结合。 每个连续的多糖多层自发地与前一层相关联。 由于在多层多层上存在相反电荷的官能团，或由于各层之间的官能团之间的自发反应，发生自发缔合。 载体可以是诸如管，微量滴定板，珠粒，颗粒等的任何表面，并且适用于诊断或治疗方法。