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    Methods for detection, identification and quantification of impurities
    1.
    发明授权
    Methods for detection, identification and quantification of impurities 失效
    检测，鉴定和定量杂质的方法

    公开(公告)号：US07871519B2

    公开(公告)日：2011-01-18

    申请号：US11910057

    申请日：2006-03-31

    申请人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-joachim Gaus ,  Claus André Frank Rentel

    发明人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-joachim Gaus ,  Claus André Frank Rentel

    IPC分类号： B01D15/08

    CPC分类号： G01N30/7233 ,  G01N30/02 ,  G01N30/34 ,  G01N2030/342 ,  G01N2030/8813 ,  G01N2030/8827 ,  B01D15/366

    摘要： Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.

    摘要翻译： 提供了一种方法，包括将包含多个寡核苷酸的样品引入具有缓冲流动相的离子对高效液相色谱柱中，并允许至少一部分寡核苷酸分离; 允许寡核苷酸从柱洗脱; 并将寡核苷酸引入质谱仪中，并通过质谱法定量至少一部分寡核苷酸。 在该方法中，寡核苷酸的至少一部分是质量不超过20％的共洗脱寡核苷酸; 并且缓冲的流动相使至少50摩尔％的共洗脱寡核苷酸在进入质谱仪时具有相同的电荷。

    METHODS FOR DETECTION, IDENTIFICATION AND QUANTIFICATION OF IMPURITIES
    2.
    发明申请
    METHODS FOR DETECTION, IDENTIFICATION AND QUANTIFICATION OF IMPURITIES 失效
    检测，识别和定量的方法

    公开(公告)号：US20090095896A1

    公开(公告)日：2009-04-16

    申请号：US11910057

    申请日：2006-03-31

    申请人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-Joachim Gaus ,  Claus Andre Frank Rental

    发明人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-Joachim Gaus ,  Claus Andre Frank Rental

    IPC分类号： H01J49/00 ,  G01N30/84

    CPC分类号： G01N30/7233 ,  G01N30/02 ,  G01N30/34 ,  G01N2030/342 ,  G01N2030/8813 ,  G01N2030/8827 ,  B01D15/366

    摘要： Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.

    摘要翻译： 提供了一种方法，包括将包含多个寡核苷酸的样品引入具有缓冲流动相的离子对高效液相色谱柱中，并允许至少一部分寡核苷酸分离; 允许寡核苷酸从柱洗脱; 并将寡核苷酸引入质谱仪中，并通过质谱法定量至少一部分寡核苷酸。 在该方法中，寡核苷酸的至少一部分是质量不超过20％的共洗脱寡核苷酸; 并且缓冲的流动相使至少50摩尔％的共洗脱寡核苷酸在进入质谱仪时具有相同的电荷。