公开(公告)号：US20060040308A1

公开(公告)日：2006-02-23

申请号：US11209160

申请日：2005-08-22

申请人： Daniel Capaldi ,  Hans-Joachim Gaus ,  Claus Rentel ,  Philip Olsen ,  Christine Kurata ,  Quanlai Song

发明人： Daniel Capaldi ,  Hans-Joachim Gaus ,  Claus Rentel ,  Philip Olsen ,  Christine Kurata ,  Quanlai Song

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07H21/04

摘要： The present invention relates to oligonucleotide synthesis. In particular, the present invention provides methods for characterizing samples useful for making oligonucleotides.

摘要翻译： 本发明涉及寡核苷酸合成。 特别地，本发明提供用于表征可用于制备寡核苷酸的样品的方法。

公开(公告)号：US20090095896A1

公开(公告)日：2009-04-16

申请号：US11910057

申请日：2006-03-31

申请人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-Joachim Gaus ,  Claus Andre Frank Rental

发明人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-Joachim Gaus ,  Claus Andre Frank Rental

IPC分类号： H01J49/00 ,  G01N30/84

CPC分类号： G01N30/7233 ,  G01N30/02 ,  G01N30/34 ,  G01N2030/342 ,  G01N2030/8813 ,  G01N2030/8827 ,  B01D15/366

摘要： Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.

摘要翻译： 提供了一种方法，包括将包含多个寡核苷酸的样品引入具有缓冲流动相的离子对高效液相色谱柱中，并允许至少一部分寡核苷酸分离; 允许寡核苷酸从柱洗脱; 并将寡核苷酸引入质谱仪中，并通过质谱法定量至少一部分寡核苷酸。 在该方法中，寡核苷酸的至少一部分是质量不超过20％的共洗脱寡核苷酸; 并且缓冲的流动相使至少50摩尔％的共洗脱寡核苷酸在进入质谱仪时具有相同的电荷。

公开(公告)号：US07871519B2

公开(公告)日：2011-01-18

申请号：US11910057

申请日：2006-03-31

申请人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-joachim Gaus ,  Claus André Frank Rentel

发明人： Christine Kurata ,  Daniel Capaldi ,  Zhiwei Wang ,  Nhuy Luu ,  Hans-joachim Gaus ,  Claus André Frank Rentel

IPC分类号： B01D15/08

CPC分类号： G01N30/7233 ,  G01N30/02 ,  G01N30/34 ,  G01N2030/342 ,  G01N2030/8813 ,  G01N2030/8827 ,  B01D15/366

摘要： Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.

摘要翻译： 提供了一种方法，包括将包含多个寡核苷酸的样品引入具有缓冲流动相的离子对高效液相色谱柱中，并允许至少一部分寡核苷酸分离; 允许寡核苷酸从柱洗脱; 并将寡核苷酸引入质谱仪中，并通过质谱法定量至少一部分寡核苷酸。 在该方法中，寡核苷酸的至少一部分是质量不超过20％的共洗脱寡核苷酸; 并且缓冲的流动相使至少50摩尔％的共洗脱寡核苷酸在进入质谱仪时具有相同的电荷。