DNA cassette for cellular expression of small RNA
    1.
    发明申请
    DNA cassette for cellular expression of small RNA 审中-公开
    用于细胞表达小RNA的DNA盒

    公开(公告)号:US20050196862A1

    公开(公告)日:2005-09-08

    申请号:US10652971

    申请日:2003-08-29

    IPC分类号: A61K48/00 C12N15/85

    摘要: We describe expression cassettes and processes for preparing expression cassettes that can be delivered to animal cells in vivo or in vitro. Delivery of the cassettes results in expression of small RNA transcripts such as siRNA in the cell. The cassettes can by used to inhibit gene expression in the cell and to screen siRNA and other RNA sequences for functional efficacy.

    摘要翻译: 我们描述了用于制备可在体内或体外递送至动物细胞的表达盒的表达盒和方法。 盒的递送导致细胞中小RNA转录物如siRNA的表达。 盒可以用于抑制细胞中的基因表达,并筛选siRNA和其他RNA序列的功能功效。