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1.发明授权Enhancing intermolecular integration of nucleic acids using integrator complexes 有权使用积分复合物增强核酸的分子间整合公开(公告)号：US07262056B2
公开(公告)日：2007-08-28
申请号：US10291342
申请日：2002-11-08
申请人： Christine Wooddell , Hans Herweijer , Jon A. Wolff
发明人： Christine Wooddell , Hans Herweijer , Jon A. Wolff
IPC分类号： C12N15/00 , C12N15/74 , C12N15/861 , C12N15/66 , C12N1/20
CPC分类号： C12N9/22 , C12N15/907 , C12N2800/90 , C12N2840/20
摘要： We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is enhanced by cationic reagents.
摘要翻译： 我们公开了用于增强转座子介导的核酸分子整合到另一靶核酸分子中的组合物和方法。 通过阳离子试剂增强了积分器复合物的整合。
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公开(公告)号：US20050196862A1
公开(公告)日：2005-09-08
申请号：US10652971
申请日：2003-08-29
申请人： Christine Wooddell , Hans Herweijer , Jon Wolff
发明人： Christine Wooddell , Hans Herweijer , Jon Wolff
CPC分类号： C12N15/85 , C12N15/111 , C12N2310/14 , C12N2330/51 , C12N2310/531
摘要： We describe expression cassettes and processes for preparing expression cassettes that can be delivered to animal cells in vivo or in vitro. Delivery of the cassettes results in expression of small RNA transcripts such as siRNA in the cell. The cassettes can by used to inhibit gene expression in the cell and to screen siRNA and other RNA sequences for functional efficacy.
摘要翻译： 我们描述了用于制备可在体内或体外递送至动物细胞的表达盒的表达盒和方法。 盒的递送导致细胞中小RNA转录物如siRNA的表达。 盒可以用于抑制细胞中的基因表达，并筛选siRNA和其他RNA序列的功能功效。
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公开(公告)号：US07101995B2
公开(公告)日：2006-09-05
申请号：US10157674
申请日：2002-05-28
申请人： David L. Lewis , James E. Hagstrom , Hans Herweijer , Aaron G. Loomis , Sean D. Monahan , Jon A. Wolff
发明人： David L. Lewis , James E. Hagstrom , Hans Herweijer , Aaron G. Loomis , Sean D. Monahan , Jon A. Wolff
CPC分类号： C12N15/1137 , A61K31/58 , A61K31/74 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/88 , C12N2310/14 , C12N2320/32 , C12N2710/24111 , C12Y206/01002 , A61K2300/00
摘要： Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
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公开(公告)号：US07803782B2
公开(公告)日：2010-09-28
申请号：US10855175
申请日：2004-05-27
申请人： James E. Hagstrom , Julia Hegge , Hans Herweijer , Jon A. Wolff
发明人： James E. Hagstrom , Julia Hegge , Hans Herweijer , Jon A. Wolff
CPC分类号： A61K48/0083 , A61K48/0075
摘要： An intravenous delivery method is described that enables delivery of polynucleotides to extravascular cells of a mammalian limb. The method involves the injection of polynucleotides into a distal vein of a limb that is transiently occluded. Polynucleotide delivery is facilitated by rapid injection in sufficient volume to enable extravasation of the solution into surrounding tissue.
摘要翻译： 描述了能够将多核苷酸递送到哺乳动物肢体的血管外细胞的静脉内递送方法。 该方法包括将多核苷酸注射入瞬时闭塞的肢体的远端静脉。 通过快速注射足够的体积来促进多核苷酸递送以使溶液能够外渗到周围的组织中。
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5.发明授权Compositions and processes using siRNA, amphipathic compounds and polycations 有权使用siRNA，两亲性化合物和聚阳离子的组合物和方法公开(公告)号：US07601367B2
公开(公告)日：2009-10-13
申请号：US10845968
申请日：2004-05-14
申请人： Sean D. Monahan , David L. Lewis , Hans Herweijer , Jon A. Wolff , James E. Hagstrom , Aaron G. Loomis , Vladimir Trubetskoy , Lori Higgs
发明人： Sean D. Monahan , David L. Lewis , Hans Herweijer , Jon A. Wolff , James E. Hagstrom , Aaron G. Loomis , Vladimir Trubetskoy , Lori Higgs
CPC分类号： C12N15/111 , A61K31/495 , A61K31/58 , A61K31/7105 , A61K31/74 , A61K48/0025 , A61K48/0041 , C07H21/04 , C12N15/88 , C12N2310/14 , C12N2320/32 , A61K2300/00
摘要： Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
摘要翻译： 描述了具有低毒性的可递送组合物，其包含两亲性化合物，聚阳离子和siRNA。 组合物可以用于将siRNA递送到动物细胞或更特别是哺乳动物细胞的过程中。
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公开(公告)号：US20080152661A1
公开(公告)日：2008-06-26
申请号：US11840468
申请日：2007-08-17
申请人： David B. Rozema , Darren H. Wakefield , Jason Klein , So Wong , Jon A. Wolff , David L. Lewis , James E. Hagstrom , Andrei Blokhin , Vladimir Trubetskoy , Hans Herweijer
发明人： David B. Rozema , Darren H. Wakefield , Jason Klein , So Wong , Jon A. Wolff , David L. Lewis , James E. Hagstrom , Andrei Blokhin , Vladimir Trubetskoy , Hans Herweijer
IPC分类号： A61K31/70 , A61K39/395 , C12N5/00 , A61K38/00
CPC分类号： C12N15/87 , A01K2217/058 , A01K2267/0362 , A61K47/58 , A61K48/0041
摘要： The present invention is directed to compounds, compositions, and methods useful for delivering polynucleotides or other cell-impermeable molecules to mammalian cells. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery vehicles. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.
摘要翻译： 本发明涉及可用于将多核苷酸或其它细胞不可渗透分子递送至哺乳动物细胞的化合物，组合物和方法。 描述了将靶向，抗调理作用，抗聚集和转染活性结合到小的生物相容性体内递送载体中的多偶联体系。 连接组成部分的多个可逆连接的使用提供了生理响应活性调节。
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公开(公告)号：US07473419B2
公开(公告)日：2009-01-06
申请号：US11268276
申请日：2005-11-07
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
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公开(公告)号：US07015040B2
公开(公告)日：2006-03-21
申请号：US11008856
申请日：2004-12-10
IPC分类号： C12N15/88
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
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公开(公告)号：US06867196B1
公开(公告)日：2005-03-15
申请号：US09330909
申请日：1999-06-11
申请人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号： A61K48/0008 , A61K48/0075
摘要： A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
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公开(公告)号：US07781415B2
公开(公告)日：2010-08-24
申请号：US10770588
申请日：2004-02-03
申请人： Hans Herweijer , Julia Hegge , Jon A. Wolff , David L. Lewis
发明人： Hans Herweijer , Julia Hegge , Jon A. Wolff , David L. Lewis
IPC分类号： A61K48/00
CPC分类号： A61K48/0083
摘要： A process for delivering a polynucleotide to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a polynucleotide into a blood vessel and increasing permeability of the blood vessel to the polynucleotide. The polynucleotide can be a small interfering RNA or microRNA and inhibit gene expression in the cell.
摘要翻译： 描述了将多核苷酸递送至哺乳动物心脏组织细胞的方法，包括将由多核苷酸组成的组合物引入血管并增加血管对多核苷酸的通透性。 多核苷酸可以是小干扰RNA或微小RNA并抑制细胞中的基因表达。
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