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公开(公告)号：US5854067A
公开(公告)日：1998-12-29
申请号：US588983
申请日：1996-01-19
IPC分类号： C07K14/62 , C12N9/10 , C12N9/12 , C12N15/113 , C12N15/54 , C12N15/85 , C12N15/00 , C12N15/63 , C12Q1/68
CPC分类号： C12N15/1137 , C07K14/62 , C12N9/1051 , C12N9/1205 , C12Y207/01001 , C12N2310/121
摘要： Disclosed are compositions and methods for inhibiting hexokinase enzymes in mammalian cells. Specifically provided are proteins that stimulate the production of trehalose-6-phosphate and their respective genes; hexokinase-specific ribozymes and genes encoding such constructs; and agents that competitively reduce hexokinase activity, e.g., by displacing hexokinase from mitochondria, and their respective genes. The latter group of agents includes inactive hexokinases and fragments thereof that retain mitochondrial binding functions and hexokinase-glucokinase chimeras that further substitute glucokinase activity for hexokinase activity. Mammalian cells including such hexokinase inhibitors, methods of making such cells and various in vitro and in vivo methods of using cells with reduced hexokinase activity are also described herein.
摘要翻译： 公开了用于在哺乳动物细胞中抑制己糖激酶的组合物和方法。 具体提供的是刺激海藻糖-6-磷酸及其各自基因的产生的蛋白质; 己糖激酶特异性核酶和编码这种构建体的基因; 以及竞争性降低己糖激酶活性的试剂，例如通过从线粒体置换己糖激酶及其各自的基因。 后一组试剂包括保留线粒体结合功能的失活的己糖激酶及其片段和进一步用葡萄糖激酶活性替代己糖激酶活性的己糖激酶 - 葡糖激酶嵌合体。 本文还描述了包括这种己糖激酶抑制剂的哺乳动物细胞，制备这种细胞的方法以及使用具有降低的己糖激酶活性的细胞的各种体外和体内方法。
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公开(公告)号：US5891717A
公开(公告)日：1999-04-06
申请号：US588976
申请日：1996-01-19
CPC分类号： C12N9/1205 , C07K14/62
摘要： Disclosed are compositions and methods for inhibiting hexokinase enzymes in mammalian cells. Specifically provided are proteins that stimulate the production of trehalose-6-phosphate and their respective genes; hexokinase-specific ribozymes and genes encoding such constructs; and agents that competitively reduce hexokinase activity, e.g., by displacing hexokinase from mitochondria, and their respective genes. The latter group of agents includes inactive hexokinases and fragments thereof that retain mitochondrial binding functions and hexokinase-glucokinase chimeras that further substitute glucokinase activity for hexokinase activity. Mammalian cells including such hexokinase inhibitors, methods of making such cells and various in vitro and in vivo methods of using cells with reduced hexokinase activity are also described herein.
摘要翻译： 公开了用于在哺乳动物细胞中抑制己糖激酶的组合物和方法。 具体提供的是刺激海藻糖-6-磷酸及其各自基因的产生的蛋白质; 己糖激酶特异性核酶和编码这种构建体的基因; 以及竞争性降低己糖激酶活性的试剂，例如通过从线粒体置换己糖激酶及其各自的基因。 后一组试剂包括保留线粒体结合功能的失活的己糖激酶及其片段和进一步用葡萄糖激酶活性替代己糖激酶活性的己糖激酶 - 葡糖激酶嵌合体。 本文还描述了包括这种己糖激酶抑制剂的哺乳动物细胞，制备这种细胞的方法以及使用具有降低的己糖激酶活性的细胞的各种体外和体内方法。
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